CAJ | 학술논문

目的：测定牛蒡植株不同部位总黄酮的含量。方法：采用L9（34）正交设计试验，对提取时间、料液比、乙醇浓度、提取次数4因素进行考察，以确定最佳提取工艺；以芦丁为对照，用亚硝酸钠-硝酸铝-氢氧化钠比色法测定总黄酮含量。结果：最佳提取工艺为：乙醇浓度55%，料液比1∶70，提取时间1.5 h，提取次数2次；芦丁在4.00~100.00μg/mL范围内线性关系良好，r=0.9998，总黄酮平均回收率为97.7%，RSD为2.5%（n=6）。结论：该提取工艺稳定可行，含量测定方法准确。
목적：측정우방식주불동부위총황동적함량。방법：채용L9（34）정교설계시험，대제취시간、료액비、을순농도、제취차수4인소진행고찰，이학정최가제취공예；이호정위대조，용아초산납-초산려-경양화납비색법측정총황동함량。결과：최가제취공예위：을순농도55%，료액비1∶70，제취시간1.5 h，제취차수2차；호정재4.00~100.00μg/mL범위내선성관계량호，r=0.9998，총황동평균회수솔위97.7%，RSD위2.5%（n=6）。결론：해제취공예은정가행，함량측정방법준학。
Objective: To determine the content of the total flavones from the Arctium lappa L.. Methods: The best extraction technology was determined by orthogonal experiment design, including extraction time,solid-liquid ratio,ethanol concentration and the extraction times. The content of total flavones was determined by absorption spectrometry with rutosids as reference substance. Results: Optimum extraction technology was as follows: 55% ethanol,ratio of material to liquid 1∶70,time 1.5 h,two times. The calibration curve had good linear relationship in the range of 4.00~100.00 μg/mL. The average recovery was 97.7%,RSD=2.5%(n=6). Conclusion:The extraction process is stable and feasible. The quantitative method is accurate.