大理学院学报
大理學院學報
대이학원학보
JOURNAL OF DALI COLLEGE
2013年
12期
9-11
,共3页
杨晓珍%尹腊梅%杨永寿
楊曉珍%尹臘梅%楊永壽
양효진%윤석매%양영수
川滇桤木%总黄酮%紫外-可见分光光度法
川滇榿木%總黃酮%紫外-可見分光光度法
천전기목%총황동%자외-가견분광광도법
Alnus ferdinandi-coburgii%flavonoid%UV-visible method
目的:建立川滇桤木果实中总黄酮的含量测定方法。方法:采用紫外-可见分光光度法,以芦丁为对照品测定川滇桤木果实中总黄酮的含量。结果:芦丁在0.19~0.95 mg/mL范围内呈良好的线性关系,A=1.0826C+0.0497(r=0.9996),平均加样回收率为99.6%,RSD为1.09%(n=6)。结论:该方法操作简便、快速、准确、重现性好,可作为川滇桤木果实中总黄酮的含量测定方法。
目的:建立川滇榿木果實中總黃酮的含量測定方法。方法:採用紫外-可見分光光度法,以蘆丁為對照品測定川滇榿木果實中總黃酮的含量。結果:蘆丁在0.19~0.95 mg/mL範圍內呈良好的線性關繫,A=1.0826C+0.0497(r=0.9996),平均加樣迴收率為99.6%,RSD為1.09%(n=6)。結論:該方法操作簡便、快速、準確、重現性好,可作為川滇榿木果實中總黃酮的含量測定方法。
목적:건립천전기목과실중총황동적함량측정방법。방법:채용자외-가견분광광도법,이호정위대조품측정천전기목과실중총황동적함량。결과:호정재0.19~0.95 mg/mL범위내정량호적선성관계,A=1.0826C+0.0497(r=0.9996),평균가양회수솔위99.6%,RSD위1.09%(n=6)。결론:해방법조작간편、쾌속、준학、중현성호,가작위천전기목과실중총황동적함량측정방법。
Objective: To establish a method for determining the content of flavonoid in Alnus ferdinandi-coburgii. Methods: The content of flavonoid was detected from Alnus ferdinandi-coburgii by UV-visible method and the rutin was used as control.Results:Rutin was good linear in the range of 0.19~0.95 mg/mL:A=1.082 6C+0.049 7(r=0.999 6)the average recovery of 99.6﹪and RSD of 1.09﹪(n=6). Conclusion: The method is simple, rapid and accurate. It can be used for determining the content of total flavones in Alnus ferdinandi-coburgii.