CAJ | 학술논문

目的：探讨人永生化尿路上皮细胞系（immortalized human bladder urothelium cell line） SV-HUC-1在聚己内酯/丝素蛋白[Poly（ε-caprolactone）/silk fibroin，PCL/SF]支架中的生长情况，为进一步体内组织修复提供依据。方法制备疏松多孔的PCL/SF支架材料，SV-HUC-1传代扩增后，接种在PCL/SF上，体外培养7 d后取材，观察SV-HUC-1和PCL/SF的复合情况。结果 SV-HUC-1呈“多角形”、“铺路石”样，以克隆团形式生长。HE染色显示PCL/SF支架上的SV-HUC-1经过体外培养7 d后即可形成沿支架表面排列的复层上皮结构，部分SV-HUC-1渗透到PCL/SF的空隙内。扫描电镜显示细胞材料复合良好。结论 PCL/SF能支持SV-HUC-1良好的生长，该支架可以作为载体材料，用于组织工程膀胱修复的研究。
목적：탐토인영생화뇨로상피세포계（immortalized human bladder urothelium cell line） SV-HUC-1재취기내지/사소단백[Poly（ε-caprolactone）/silk fibroin，PCL/SF]지가중적생장정황，위진일보체내조직수복제공의거。방법제비소송다공적PCL/SF지가재료，SV-HUC-1전대확증후，접충재PCL/SF상，체외배양7 d후취재，관찰SV-HUC-1화PCL/SF적복합정황。결과 SV-HUC-1정“다각형”、“포로석”양，이극륭단형식생장。HE염색현시PCL/SF지가상적SV-HUC-1경과체외배양7 d후즉가형성연지가표면배렬적복층상피결구，부분SV-HUC-1삼투도PCL/SF적공극내。소묘전경현시세포재료복합량호。결론 PCL/SF능지지SV-HUC-1량호적생장，해지가가이작위재체재료，용우조직공정방광수복적연구。
Objective To observe the growth of the immortalized human bladder urothelium cell line (SV-HUC-1) on(Poly(ε-caprolactone)/silk fibroin, PCL/SF) scaffold in vitro. Methods Porous PCL/SF scaffolds was prepared. After in vitro culture and amplification, the SV-HUC-1were seeded onto the PCL/CS scaffolds. After cultured for 7 days, the cell-scaffolds composites were observed. Results SV-HUC-1 were polygon and paving stone-like, and they could form the clone groups. After 1 week of in vitro culture, the SV-HUC-1formed a multilayer structure on the surface of the scaffolds, and some of them grew into the scaffolds. Scanning electron microscopy shows that SV-HUC-1 and PCL/SF composited well. Conclusion PCL/SF have a good biocompatibility with SV-HUC-1, which can be used as a vehicle in bladder defect reconstruction with the application of tissue engineering.