中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
19期
8707-8710
,共4页
徐晓晗%姜贵洋%张秀鹏%苗原
徐曉晗%薑貴洋%張秀鵬%苗原
서효함%강귀양%장수붕%묘원
癌,非小细胞肺%钙黏着糖蛋白类%β连环素%p130 cas%肿瘤侵袭
癌,非小細胞肺%鈣黏著糖蛋白類%β連環素%p130 cas%腫瘤侵襲
암,비소세포폐%개점착당단백류%β련배소%p130 cas%종류침습
Carcinoma,non-small-cell lung%Cadherins%beta catenin%p130 cas%tumor invasion
目的：探讨p130 cas在非小细胞肺癌中的表达与E-cadherin/β-catenin复合体的关系及对肿瘤细胞侵袭能力的影响。方法应用免疫组化方法检测p130 cas在145例非小细胞肺癌配对癌旁正常肺组织中的表达，并分析其表达与E-cadherin及β-catenin表达的相关性；同时进一步通过siRNA技术在肺癌细胞系中下调p130 cas的表达，观察E-cadherin和β-catenin表达的变化及肺癌细胞侵袭能力的变化。结果 p130 cas在非小细胞肺癌组织标本中表达上调（P＜0.001），并与E-cadherin和β-catenin的表达下调相关（P＝0.001和P＝0.003）；Western blot结果显示在肺癌细胞系A549和H1299中下调p130 cas的表达可以使E-cadherin和β-catenin的表达上调，而且侵袭实验结果显示下调p130 cas的表达可以使肺癌细胞的侵袭能力下降。结论 p130 cas在肺癌中可能通过下调E-cadherin/β-catenin复合体的表达促进肿瘤细胞的侵袭和转移。
目的：探討p130 cas在非小細胞肺癌中的錶達與E-cadherin/β-catenin複閤體的關繫及對腫瘤細胞侵襲能力的影響。方法應用免疫組化方法檢測p130 cas在145例非小細胞肺癌配對癌徬正常肺組織中的錶達，併分析其錶達與E-cadherin及β-catenin錶達的相關性；同時進一步通過siRNA技術在肺癌細胞繫中下調p130 cas的錶達，觀察E-cadherin和β-catenin錶達的變化及肺癌細胞侵襲能力的變化。結果 p130 cas在非小細胞肺癌組織標本中錶達上調（P＜0.001），併與E-cadherin和β-catenin的錶達下調相關（P＝0.001和P＝0.003）；Western blot結果顯示在肺癌細胞繫A549和H1299中下調p130 cas的錶達可以使E-cadherin和β-catenin的錶達上調，而且侵襲實驗結果顯示下調p130 cas的錶達可以使肺癌細胞的侵襲能力下降。結論 p130 cas在肺癌中可能通過下調E-cadherin/β-catenin複閤體的錶達促進腫瘤細胞的侵襲和轉移。
목적：탐토p130 cas재비소세포폐암중적표체여E-cadherin/β-catenin복합체적관계급대종류세포침습능력적영향。방법응용면역조화방법검측p130 cas재145례비소세포폐암배대암방정상폐조직중적표체，병분석기표체여E-cadherin급β-catenin표체적상관성；동시진일보통과siRNA기술재폐암세포계중하조p130 cas적표체，관찰E-cadherin화β-catenin표체적변화급폐암세포침습능력적변화。결과 p130 cas재비소세포폐암조직표본중표체상조（P＜0.001），병여E-cadherin화β-catenin적표체하조상관（P＝0.001화P＝0.003）；Western blot결과현시재폐암세포계A549화H1299중하조p130 cas적표체가이사E-cadherin화β-catenin적표체상조，이차침습실험결과현시하조p130 cas적표체가이사폐암세포적침습능력하강。결론 p130 cas재폐암중가능통과하조E-cadherin/β-catenin복합체적표체촉진종류세포적침습화전이。
Objective To investigate the relationships between p130 cas expression and E-cadherin/β-catenin adhesive complex and to explore their effects on the invasiveness of NSCLC. Methods Immunohistochemistry was used to examine the expression of p130 cas, E-cadherin andβ-catenin in 145 lung cancer tissues and paired adjacent normal lung tissues. p130 cas specific siRNA was introduced into lung cancer cell lines, western blot and transwell were used to evaluate the changes of E-cadherin and β-catenin expression and cancer cells’ invasive capabilities. Results p130 cas expression was upregulated in NSCLC tissue samples (P<0.001). The p130 cas expression was correlated with loss of E-cadherin andβ-catenin expression (P=0.001 and P=0.003, respectively). Western blotting results showed that downregulated p130 cas expression by siRNA transfection could restore both E-cadherin andβ-catenin expression and transwell test results showed that inhibit the invasive capabilities of lung cancer cells. Conclusion These results indicate that p130 cas may facilitate invasiveness and metastasis of tumor cell through its repression on E-cadherin andβ-catenin expression in NSCLC.
