中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
19期
8573-8576
,共4页
李博%朱平先%刘强%刘宇%彭立军%刘光金%江秋兰%罗清芬
李博%硃平先%劉彊%劉宇%彭立軍%劉光金%江鞦蘭%囉清芬
리박%주평선%류강%류우%팽립군%류광금%강추란%라청분
左心室舒张功能衰竭%心室重构%核因子-κB%转化生长因子β1
左心室舒張功能衰竭%心室重構%覈因子-κB%轉化生長因子β1
좌심실서장공능쇠갈%심실중구%핵인자-κB%전화생장인자β1
Left ventricular diastolic function failure%Ventricular remodeling%Nuclear factor kappa B%Transforming growth factor beta 1
目的：探讨左心室舒张功能衰竭（DHF）患者外周血NF-κB、血清TGF-β1的水平变化及其与心室重构的相关性。方法选取150例DHF患者（实验组）和30例正常人（对照组），采用双抗体夹心酶联免疫吸附法（ELISA）检测外周血 NF-κB 和血清 TGF-β1的表达水平。利用心脏超声检测结果计算左心室心肌质量指数（LVMI）、平均室壁应力（MWS）。结果 DHF患者NF-κB、TGF-β1水平和LVMI、MWS均显著高于对照组（P＜0.01），且随着心衰程度的加重而增加（P＜0.01）；DHF患者NF-κB、TGF-β1水平与反应心室重构的指标 LVMI、MWS 正相关（P＜0.01）。多元线性回归分析显示，NF-κB、TGF-β1水平与LVMI均呈独立正相关（P＜0.01）。结论 DHF患者NF-κB、TGF-β1水平增加，与DHF患者心室重构严重程度呈独立、显著的相关性。
目的：探討左心室舒張功能衰竭（DHF）患者外週血NF-κB、血清TGF-β1的水平變化及其與心室重構的相關性。方法選取150例DHF患者（實驗組）和30例正常人（對照組），採用雙抗體夾心酶聯免疫吸附法（ELISA）檢測外週血 NF-κB 和血清 TGF-β1的錶達水平。利用心髒超聲檢測結果計算左心室心肌質量指數（LVMI）、平均室壁應力（MWS）。結果 DHF患者NF-κB、TGF-β1水平和LVMI、MWS均顯著高于對照組（P＜0.01），且隨著心衰程度的加重而增加（P＜0.01）；DHF患者NF-κB、TGF-β1水平與反應心室重構的指標 LVMI、MWS 正相關（P＜0.01）。多元線性迴歸分析顯示，NF-κB、TGF-β1水平與LVMI均呈獨立正相關（P＜0.01）。結論 DHF患者NF-κB、TGF-β1水平增加，與DHF患者心室重構嚴重程度呈獨立、顯著的相關性。
목적：탐토좌심실서장공능쇠갈（DHF）환자외주혈NF-κB、혈청TGF-β1적수평변화급기여심실중구적상관성。방법선취150례DHF환자（실험조）화30례정상인（대조조），채용쌍항체협심매련면역흡부법（ELISA）검측외주혈 NF-κB 화혈청 TGF-β1적표체수평。이용심장초성검측결과계산좌심실심기질량지수（LVMI）、평균실벽응력（MWS）。결과 DHF환자NF-κB、TGF-β1수평화LVMI、MWS균현저고우대조조（P＜0.01），차수착심쇠정도적가중이증가（P＜0.01）；DHF환자NF-κB、TGF-β1수평여반응심실중구적지표 LVMI、MWS 정상관（P＜0.01）。다원선성회귀분석현시，NF-κB、TGF-β1수평여LVMI균정독립정상관（P＜0.01）。결론 DHF환자NF-κB、TGF-β1수평증가，여DHF환자심실중구엄중정도정독립、현저적상관성。
Objective To investigate the expression changes of peripheral blood NF-κB and serum TGF-β1 in patients with left ventricular diastolic dysfunction(DHF) and explore their correlation with ventricular remodeling. Methods 150 patients diagnosed with DHF were recruited as the experimental group and 30 normal subjects were selected as control group. A double-antibody sandwich enzyme-linked immunosorbent assay(ELISA) was used to detect the peripheral blood NF-κB level and serum TGF-β1 level. Echocardiography was employed. Furthermore, left ventricular mass index (LVMI) and left ventricular mean wall stress (MWS) were calculated. Results Compared with the control group, the NF-κB levels, TGF-β1 levels, LVMI and MWS were significantly higher in DHF patients(P<0.01), and the NF-κB levels, TGF-β1 levels, LVMI and MWS rose correspondingly when cardiac function worsened(P<0.01). The NF-κB and TGF-β1 levels in DHF patients were positively correlated with LVMI and MWS(P<0.01). The positive association between NF-κB and LVMI, TGF-β1 and LVMI remained significant in a multivariate regression model(P<0.01). Conclusion Higher levels of peripheral blood NF-κB and serum TGF-β1 in DHF patients are significantly correlated with the severity of ventricular remodeling.
