中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
19期
8565-8568
,共4页
曹慧%谭善忠%孙薇薇%沈建军%肖倩%梁重锋%蒋淑莲
曹慧%譚善忠%孫薇薇%瀋建軍%肖倩%樑重鋒%蔣淑蓮
조혜%담선충%손미미%침건군%초천%량중봉%장숙련
肝功能衰竭%乙型肝炎病毒相关慢加急性肝衰竭%T淋巴细胞%T淋巴细胞,调节性%特征
肝功能衰竭%乙型肝炎病毒相關慢加急性肝衰竭%T淋巴細胞%T淋巴細胞,調節性%特徵
간공능쇠갈%을형간염병독상관만가급성간쇠갈%T림파세포%T림파세포,조절성%특정
Liver failure%Hepatitis B virus-related acute-on-chronic liver failure%T lymphocytes%T-lymphocytes,regulatory%Feature
目的探讨乙型肝炎病毒相关慢加急性肝衰竭患者外周血T淋巴细胞的变化及意义。方法乙型肝炎病毒相关慢加急性肝衰竭患者（肝衰竭组）与慢性乙型肝炎患者（慢乙肝组）各50例，以门诊同期健康体检者（健康对照组）20名为对照，应用流式细胞仪测定外周血CD3＋T淋巴细胞所占比例、CD4＋和CD8＋T淋巴细胞所占比例及比值、CD4＋CD25＋调节性T淋巴细胞所占比例。结果肝衰竭组外周血CD3＋T淋巴细胞[（35.48±23.44）%]、CD8＋T淋巴细胞[（37.66±13.28）%]、CD4＋CD25＋调节性T淋巴细胞[（0.72±1.07）%]所占比例与健康对照组[分别为（50.31±12.09）%、（42.05±9.26）%、（2.93±1.31）%]和慢乙肝组[分别为（49.72±20.11）%、（41.95±8.63）%、（3.47±2.29）%]比较均下降，差异有统计学意义（P＜0.05）。结论乙型肝炎病毒相关慢加急性肝衰竭一旦形成，有别于慢性乙型肝炎时期，外周血总T淋巴细胞、CD8＋T淋巴细胞以及CD4＋CD25＋调节性T淋巴细胞等效应T淋巴细胞便处于耗损状态。
目的探討乙型肝炎病毒相關慢加急性肝衰竭患者外週血T淋巴細胞的變化及意義。方法乙型肝炎病毒相關慢加急性肝衰竭患者（肝衰竭組）與慢性乙型肝炎患者（慢乙肝組）各50例，以門診同期健康體檢者（健康對照組）20名為對照，應用流式細胞儀測定外週血CD3＋T淋巴細胞所佔比例、CD4＋和CD8＋T淋巴細胞所佔比例及比值、CD4＋CD25＋調節性T淋巴細胞所佔比例。結果肝衰竭組外週血CD3＋T淋巴細胞[（35.48±23.44）%]、CD8＋T淋巴細胞[（37.66±13.28）%]、CD4＋CD25＋調節性T淋巴細胞[（0.72±1.07）%]所佔比例與健康對照組[分彆為（50.31±12.09）%、（42.05±9.26）%、（2.93±1.31）%]和慢乙肝組[分彆為（49.72±20.11）%、（41.95±8.63）%、（3.47±2.29）%]比較均下降，差異有統計學意義（P＜0.05）。結論乙型肝炎病毒相關慢加急性肝衰竭一旦形成，有彆于慢性乙型肝炎時期，外週血總T淋巴細胞、CD8＋T淋巴細胞以及CD4＋CD25＋調節性T淋巴細胞等效應T淋巴細胞便處于耗損狀態。
목적탐토을형간염병독상관만가급성간쇠갈환자외주혈T림파세포적변화급의의。방법을형간염병독상관만가급성간쇠갈환자（간쇠갈조）여만성을형간염환자（만을간조）각50례，이문진동기건강체검자（건강대조조）20명위대조，응용류식세포의측정외주혈CD3＋T림파세포소점비례、CD4＋화CD8＋T림파세포소점비례급비치、CD4＋CD25＋조절성T림파세포소점비례。결과간쇠갈조외주혈CD3＋T림파세포[（35.48±23.44）%]、CD8＋T림파세포[（37.66±13.28）%]、CD4＋CD25＋조절성T림파세포[（0.72±1.07）%]소점비례여건강대조조[분별위（50.31±12.09）%、（42.05±9.26）%、（2.93±1.31）%]화만을간조[분별위（49.72±20.11）%、（41.95±8.63）%、（3.47±2.29）%]비교균하강，차이유통계학의의（P＜0.05）。결론을형간염병독상관만가급성간쇠갈일단형성，유별우만성을형간염시기，외주혈총T림파세포、CD8＋T림파세포이급CD4＋CD25＋조절성T림파세포등효응T림파세포편처우모손상태。
Objective To investigate the changes and significance of the peripheral blood T lymphocytes for patients with hepatitis B virus-related acute-on-chronic liver failure. Methods 50 patients with hepatitis B virus-related acute-on-chronic liver failure (liver failure group), 50 patients with chronic hepatitis B (chronic hepatitis B group), and 20 healthy persons taking physical examination in the corresponding period (healthy control group) were selected to determine the proportions of CD3+, CD4+, CD8+T lymphocytes and CD4+CD25+regulatory T cells (regulatory T cells, Tregs) in peripheral blood by flow cytometry. Results The proportions of the peripheral blood CD3+(35.48%±23.44%), CD8+ T lymphocytes(37.66%±13.28%) and CD4+CD25+ Tregs (0.72%±1.07%) of the patients with hepatitis B virus-related acute-on-chronic liver failure were significantly lower than that of the healthy control group (respectively 50.31%±12.09%;42.05%±9.26%;2.93%±1.31%) and chronic hepatitis B group (respectively 49.72%± 20.11%; 41.95%± 8.63%; 3.47%± 2.29%) (P<0.05). Conclusion Once the hepatitis B virus-related acute-on-chronic liver failure formed, the total peripheral blood T lymphocytes, CD8+ T lymphocytes, CD4+CD25+ Tregs and other effector cells would be in the “wear and tear”state.
