CAJ | 학술논문

[目的]探讨脊髓γ-氨基丁酸转运体-1（GAT-1）在大鼠慢性背根神经节压迫（CCD）神经病理性疼痛中的作用。[方法]选择雄性SD大鼠72只，随机分为假手术组（A组，n＝24）、CCD组（B组，n ＝24）、鞘内NO-711＋CCD组（ C组，n ＝24），三组再按不同时段分为6个亚组（ n ＝4），分别于给药前（T0）、给药后1 h （T1）、4 h（T2）、8 h（T3）、16 h（T4）、24 h（T5）时间点处死大鼠取脊髓腰段进行GAT-1蛋白Western Blot实验。[结果]与假手术组相比，B组T0、T1、T2、T3、T4、T5时间点的GAT-1蛋白含量明显增加（ P ＜0．01），鞘内注射生理盐水后，B组的GAT-1蛋白含量无明显改变（ P ＞0．05）；与B组和T0相比，鞘内注射20μg NO-711后T1、T2、T3，C组的GAT-1蛋白含量显著降低（ P ＜0．01），鞘内给药后T4，GAT-1蛋白含量逐渐回升，与B组同一时间点比GAT-1蛋白含量仍明显降低（ P ＜0．05），至给药后 T5 GAT-1蛋白含量回升到正常水平（ P ＞0．05）。[结论]CCD模型大鼠脊髓 GAT-1蛋白表达水平上调，GAT 抑制剂 NO-711能明显抑制CCD模型大鼠脊髓GA T-1蛋白表达水平的上调，但是不能抑制GA T-1蛋白基础表达水平。大鼠脊髓背角GA T-1蛋白表达水平的改变与大鼠CCD神经病理性疼痛的机制可能密切相关。
[목적]탐토척수γ-안기정산전운체-1（GAT-1）재대서만성배근신경절압박（CCD）신경병이성동통중적작용。[방법]선택웅성SD대서72지，수궤분위가수술조（A조，n＝24）、CCD조（B조，n ＝24）、초내NO-711＋CCD조（ C조，n ＝24），삼조재안불동시단분위6개아조（ n ＝4），분별우급약전（T0）、급약후1 h （T1）、4 h（T2）、8 h（T3）、16 h（T4）、24 h（T5）시간점처사대서취척수요단진행GAT-1단백Western Blot실험。[결과]여가수술조상비，B조T0、T1、T2、T3、T4、T5시간점적GAT-1단백함량명현증가（ P ＜0．01），초내주사생리염수후，B조적GAT-1단백함량무명현개변（ P ＞0．05）；여B조화T0상비，초내주사20μg NO-711후T1、T2、T3，C조적GAT-1단백함량현저강저（ P ＜0．01），초내급약후T4，GAT-1단백함량축점회승，여B조동일시간점비GAT-1단백함량잉명현강저（ P ＜0．05），지급약후 T5 GAT-1단백함량회승도정상수평（ P ＞0．05）。[결론]CCD모형대서척수 GAT-1단백표체수평상조，GAT 억제제 NO-711능명현억제CCD모형대서척수GA T-1단백표체수평적상조，단시불능억제GA T-1단백기출표체수평。대서척수배각GA T-1단백표체수평적개변여대서CCD신경병이성동통적궤제가능밀절상관。
[Objective] To explore the role of gamma-aminobutyric acid tranporter-1(GAT-1) in neuro-pathic pain caused by chronic compression of dorsal root ganglion(CCD) in rats .[Methods]A total of 72 male SD rats were randomly divided into sham group(group A ,n=24) ,CCD group(group B ,n=24) and intrathe-cal NO-711+CCD group(group C ,n =24) .According to different time ,three groups were divided into six sub-groups( n=4) .All rats were sacrificed before administration (T0 ) ,1h(T1 ) ,4h(T2 ) ,8h(T3 ) ,16h(T4 ) and 24h(T5 ) after administration .Lumbar spinal cord of rats was collected for Western Blot test of GAT-1 protein .[Results] Compared with sham group ,GAT-1 protein in group B at T0 ,T1 ,T2 ,T3 ,T4 and T5 were obviously increased( P<0 .01) .After intrathecal injection of normal saline ,GAT-1 protein in group B had no obvious change( P>0 .05) .Compared with group B and T0 ,GAT-1 protein in group C at T1 ,T2 and T3 after intrathecal injection with 20 μg NO-711 were markedly decreased ( P <0 .01) .GAT-1 protein was gradually increased at T4 after intrathecal administration .Compared with group B ,GAT-1 protein in group C was still obviously decreased at the same time point( P <0 .05) .GAT-1 protein returned to the normal level until T5 after administration( P>0 .05) .[Conclusion] The expression levels of GAT-1 protein in rat model with CCD are increased .GAT inhibitor NO-711 can obviously inhibit the up-regulation of GAT-1 protein in spine cord of rat model with CCD ,but not inhibit the basic expression level of GAT-1 .The change of GAT-1 protein of spi-nal dorsal horn in rats may be closely correlated with the mechanism of nruropathic pain in rats with CCD .