CAJ | 학술논문

目的：建立高效液相色谱法测定恩替卡韦对映异构体的方法。方法：色谱柱为CHIRALPAK AD-H (250mm×4.6mm，5μm)，以正己烷-异丙醇-无水乙醇-三氟乙酸（70：10：20：0.5）为流动相，流速为0.8ml﹒min-1，检测波长为254nm。结果：在本方法中，恩替卡韦的线性关系良好，r为0.9999；专属性强，恩替卡韦与对映异构体之间的分离度良好；精密度和稳定性良好，RSD均小于2%。结论：该方法准确、简便、快速，可用于恩替卡韦的对映异构体测定。
목적：건립고효액상색보법측정은체잡위대영이구체적방법。방법：색보주위CHIRALPAK AD-H (250mm×4.6mm，5μm)，이정기완-이병순-무수을순-삼불을산（70：10：20：0.5）위류동상，류속위0.8ml﹒min-1，검측파장위254nm。결과：재본방법중，은체잡위적선성관계량호，r위0.9999；전속성강，은체잡위여대영이구체지간적분리도량호；정밀도화은정성량호，RSD균소우2%。결론：해방법준학、간편、쾌속，가용우은체잡위적대영이구체측정。
Objective To establish a method for the enantiomers of Entecavirby HPLC.Methods The determination was carried out on CHI-RALPAK AD-H,with Hexane- Isopropanol -ethanol -trifluoroacetic acid (70:10::30: 0.5) as the mobile phase at the flow rate of 0.8ml?min-1 .The detection wavelength was 254nm. Results The method showed good linearity with a correlation coefficient (r) of 0.9999; the precision and stability were satisfactory with all RSDs below 2%. Conclusion This method is an accurate,fast and simple method for the enantiomers of Ente-cavirtablets.