医学检验与临床
醫學檢驗與臨床
의학검험여림상
MEDICAL LATORATORY SCIENCE AND CLINICES
2014年
2期
35-37
,共3页
颗粒增强免疫比浊法%同型半胱氨酸%心血管疾病%方法学评价
顆粒增彊免疫比濁法%同型半胱氨痠%心血管疾病%方法學評價
과립증강면역비탁법%동형반광안산%심혈관질병%방법학평개
Particle Enhanced Immunoturbidimetric%Homocyteine%Cardiovascular disease%Evaluation test of methodology
目的：对胶乳颗粒免疫比浊法测定血清同型半胱氨酸（HCY）的方法学进行初步评价。方法：应用胶乳颗粒免疫比浊法测定HCY的精密度、标准曲线、干扰实验、稳定性实验、并与循环酶法测定血清HCY的结果进行相关性分析。结果：颗粒增强免疫比浊法测定HCY的精密度（批内CV为1.87%、0.75%，批间CV为2.29%、5.35%）较高；HCY浓度在0μmol/L~50μmol/L范围内线性良好；当抗坏血酸浓度1107mg/L、胆红素浓度445 umol/L、血红蛋白浓度6.7 g/L；甘油三酯浓度14.6 mmol/L、氨离子浓度75 umol/L时均未对测定结果产生干扰；试剂开瓶后稳定周期也可达到25天；颗粒增强免疫比浊法和循环酶法测定HCY相关性良好（y =0.9904x +0.0813，r=0.9981）。结论：颗粒增强免疫比浊法测定血清HCY具有较高的精密度，结果准确可靠，适合临床实验室推广。
目的：對膠乳顆粒免疫比濁法測定血清同型半胱氨痠（HCY）的方法學進行初步評價。方法：應用膠乳顆粒免疫比濁法測定HCY的精密度、標準麯線、榦擾實驗、穩定性實驗、併與循環酶法測定血清HCY的結果進行相關性分析。結果：顆粒增彊免疫比濁法測定HCY的精密度（批內CV為1.87%、0.75%，批間CV為2.29%、5.35%）較高；HCY濃度在0μmol/L~50μmol/L範圍內線性良好；噹抗壞血痠濃度1107mg/L、膽紅素濃度445 umol/L、血紅蛋白濃度6.7 g/L；甘油三酯濃度14.6 mmol/L、氨離子濃度75 umol/L時均未對測定結果產生榦擾；試劑開瓶後穩定週期也可達到25天；顆粒增彊免疫比濁法和循環酶法測定HCY相關性良好（y =0.9904x +0.0813，r=0.9981）。結論：顆粒增彊免疫比濁法測定血清HCY具有較高的精密度，結果準確可靠，適閤臨床實驗室推廣。
목적：대효유과립면역비탁법측정혈청동형반광안산（HCY）적방법학진행초보평개。방법：응용효유과립면역비탁법측정HCY적정밀도、표준곡선、간우실험、은정성실험、병여순배매법측정혈청HCY적결과진행상관성분석。결과：과립증강면역비탁법측정HCY적정밀도（비내CV위1.87%、0.75%，비간CV위2.29%、5.35%）교고；HCY농도재0μmol/L~50μmol/L범위내선성량호；당항배혈산농도1107mg/L、담홍소농도445 umol/L、혈홍단백농도6.7 g/L；감유삼지농도14.6 mmol/L、안리자농도75 umol/L시균미대측정결과산생간우；시제개병후은정주기야가체도25천；과립증강면역비탁법화순배매법측정HCY상관성량호（y =0.9904x +0.0813，r=0.9981）。결론：과립증강면역비탁법측정혈청HCY구유교고적정밀도，결과준학가고，괄합림상실험실추엄。
Objective:To evaluate the detection of serum homocysteine ( HCY) with Particle Enhanced Immunoturbidimetric. Methods:The precision, calibration curve, Anti interference test,on-board stability studies,and the determination of serum HCY and enzymatic cycling assay results by correlation analysis.Results: The Particle Enhanced Immunoturbidimetric method revealed good precision ( CV 1.87%、0.75% , 2.29%、5.35%) .The linear range is0μmol/L~ 50μmol/L.The assay demonstrated less than 10% interference deviation in the presence of Vic-C(1107mg/L), bilirubin(445 umol/L), hemoglobin( 6.7 g/L),TG(14.6 mmol/L), ammoniumion(75 umol/L).samples can be stable after 25 days on-board stability.the Particle enhanced immunoturbidimetric method showed a good correlation with enzymatic cycling assay (y = 0.9904x +0.0813,r= 0.9981).Conclusions:The Particle enhanced immunoturbidimetric method is precise, reliable and suitable for determinations of serum HCY concentration in a clinical laboratory.
