CAJ | 학술논문

目的：观察石斛合剂对糖尿病模型大鼠高糖负荷后胰高血糖素样肽-1（GLP-1）影响。方法：选取雌性Wistar大鼠随机分为正常对照组（n＝10）和观察组（n＝30），观察组予高脂高糖饲料喂养4周后腹腔注射小剂量链脲佐菌素（30mg/kg）造模，成模后随机分为模型组（n＝10）、石斛合剂组（n＝10）、二甲双胍组（n＝10）。灌胃8周后，禁食10h行口服葡萄糖耐量（OGTT）试验，尾尖采血，用ELISA法检测血清GLP-1水平。结果：治疗8周后，与模型组比较，石斛合剂组的血糖降低（P<0.01），血清基础（0h）GLP-1水平明显升高（P<0.01），高糖负荷1h后，各组大鼠血清GLP-1水平均升高，石斛合剂组高于模型组与二甲双胍组（P<0.05），且与正常对照组无显著差异；高糖负荷2h后，石斛合剂组、正常对照组大鼠血清GLP-1水平均回落（组内比较，P<0.01），模型组与二甲双胍组则继续上升，各组间比较无差异；高糖负荷3h后，各组大鼠血清GLP-1水平均降低，石斛合剂组血清GLP-1水平较模型组高。结论：石斛合剂能有效的降低糖尿病模型大鼠血糖，增加血清GLP-1水平，其降糖机制可能是通过GLP-1及其降解产物信号途径介导。
목적：관찰석곡합제대당뇨병모형대서고당부하후이고혈당소양태-1（GLP-1）영향。방법：선취자성Wistar대서수궤분위정상대조조（n＝10）화관찰조（n＝30），관찰조여고지고당사료위양4주후복강주사소제량련뇨좌균소（30mg/kg）조모，성모후수궤분위모형조（n＝10）、석곡합제조（n＝10）、이갑쌍고조（n＝10）。관위8주후，금식10h행구복포도당내량（OGTT）시험，미첨채혈，용ELISA법검측혈청GLP-1수평。결과：치료8주후，여모형조비교，석곡합제조적혈당강저（P<0.01），혈청기출（0h）GLP-1수평명현승고（P<0.01），고당부하1h후，각조대서혈청GLP-1수평균승고，석곡합제조고우모형조여이갑쌍고조（P<0.05），차여정상대조조무현저차이；고당부하2h후，석곡합제조、정상대조조대서혈청GLP-1수평균회락（조내비교，P<0.01），모형조여이갑쌍고조칙계속상승，각조간비교무차이；고당부하3h후，각조대서혈청GLP-1수평균강저，석곡합제조혈청GLP-1수평교모형조고。결론：석곡합제능유효적강저당뇨병모형대서혈당，증가혈청GLP-1수평，기강당궤제가능시통과GLP-1급기강해산물신호도경개도。
Objetctive:To observe the effect of Dendrobium compound on serum glucagon-like peptide 1(GLP-1) level in type 2 diabetic rats after high sugar load. Methods:Select female Wistar rats in addition to the normal control group (n=10), give high sugar and high fat diet plus small dose of chain urea with streptozotocin (30mg/kg) to build mold, then randomly divided into model group (n=10), Dendrobium compound group, metformin group (n=10), respectively given saline, Dendrobium compound, metformin for 8 weeks. Fasted for 10h then take oral glucose tolerance test (OGTT), and serum GLP-1 level at each time point were also tested by ELISA. Result:After 8 weeks of treatment, compared with the model group, the BG level of Dendrobium compound group decreased (P<0.01), the level of GLP-1(0h) increased (P<0.01); after high sugar load (1h), the serum GLP-1 levels all increased, compared with model group and metformin group, the serum GLP-1 level of Dendrobium compound group increased (P<0.05), and no significant difference with the control group. After high sugar load (2h), Dendrobium compound group and normal control group serum GLP-1 levels were fallen back (within-group comparisons, P<0.01), and model group and metformin group continued to rise. After high sugar load (3h), the serum GLP-1 levels of all groups decreased. Conclusion:Dendrobium compound can significantly lower blood glucose, increase serum levels of GLP-1, its hypoglycemic mechanism may be mediated by glucagon-like peptide-1and its degradation product signaling pathway.