CAJ | 학술논문

目的：观察性别决定区 Y 框蛋白2（Sox2）对表皮生长因子受体（EGFR）的调控及 Sox2对胶质瘤细胞成球率的影响。方法构建表皮生长因子同源受体 ErbB2、ErbB3、ErbB4启动子报告质粒，并用双报告基因检测分析方法检测 Sox2对 ErbB2、ErbB3、ErbB4表达的调控；Sox2表达质粒转染胶质瘤 U251细胞，采用悬浮成球的方法培养 U251细胞并计数成球率，观察 U251细胞的自我复制；蛋白免疫印迹法（Western blot）检测 EGFR 和 ErbB2蛋白的表达情况。结果 Sox2可以增加悬浮培养 U251细胞的成球率（P＜0．05）；上调 ErbB2、ErbB3、ErbB4启动子介导的荧光素酶蛋白表达活性，且表现出剂量依赖性。此外，Sox2可以提高 EGFR 和 ErbB2受体的表达。结论 Sox2可以上调 EGFR 受体并促进胶质瘤干细胞的自我复制。
목적：관찰성별결정구 Y 광단백2（Sox2）대표피생장인자수체（EGFR）적조공급 Sox2대효질류세포성구솔적영향。방법구건표피생장인자동원수체 ErbB2、ErbB3、ErbB4계동자보고질립，병용쌍보고기인검측분석방법검측 Sox2대 ErbB2、ErbB3、ErbB4표체적조공；Sox2표체질립전염효질류 U251세포，채용현부성구적방법배양 U251세포병계수성구솔，관찰 U251세포적자아복제；단백면역인적법（Western blot）검측 EGFR 화 ErbB2단백적표체정황。결과 Sox2가이증가현부배양 U251세포적성구솔（P＜0．05）；상조 ErbB2、ErbB3、ErbB4계동자개도적형광소매단백표체활성，차표현출제량의뢰성。차외，Sox2가이제고 EGFR 화 ErbB2수체적표체。결론 Sox2가이상조 EGFR 수체병촉진효질류간세포적자아복제。
Objective To observe the regulation of sry-related high-mobility-group box-containing 2 (Sox2) on epidermal growth factor receptor(EGFR)and on the sphere formation rate of glioma stem cells .Methods Promoter reporter plasmids of epidermal growth factor homologous receptor ErbB2 ,ErbB3 ,ErbB4 were respectively constructed .Sox2 expression plasmid was co-transfected together with the reporter plasmid into U251 cells .Then the luciferase activity was analyzed by dual-luciferase reporter assay sys-tem to test the regulation of Sox2 on ErbB2 ,ErbB3 ,ErbB4 promoters .Sphere formation assay was used to observe selfrenew of the cancer stem cells after transfection of Sox 2 .The expression of EGFR and ErbB2 proteins in the spheres was examined by Western blot .Results Sox2 could dose-dependently increase the ErbB2 ,ErbB3 ,ErbB4 promoter drived luciferase activity .Sox2 promotes the sphere-forming rate of U251 cells and upregulates the expression of EGFR and ErbB2 in the spheres .Conclusion Sox2 could up-regulate the expression of EGFR and promote selfrenew of glioma stem cells .