CAJ | 학술논문

目的：探讨富血小板血浆（PRP）在促进兔烫伤创面愈合碱性成纤维细胞生长因子（bFGF）、血管内皮生长因子（VEGF）的表达及意义。方法将24只大白兔分为对照组和实验组，对照组用磺胺嘧啶银（SD-Ag ），实验组用PRP。在7、10、14 d ，两组分别随机选取4只大白兔麻醉处死后取材，行创面愈合率比较、H E染色及免疫组织化学检测。结果7、10、14 d实验组创面愈合率均较对照组高（ P＜0．05）。7 d时，实验组肉芽组织灶显著，细胞及血管数量密集。10 d时，实验组可见毛细血管量及成纤维细胞较对照组更为丰富。14 d时，实验组显示大部分成纤维细胞转化为纤维细胞而毛细血管数减少。7、10 d bFGF及VEGF阳性细胞数两组均增高，实验组明显高于对照组（P＜0．05），而14 d bFGF及 VEGF阳性细胞数两组均逐步下降（P＞0．05）。结论 PRP可以促进大白兔烫伤创面愈合，主要机制是明显增加早期创面中bFGF及VEGF表达。
목적：탐토부혈소판혈장（PRP）재촉진토탕상창면유합감성성섬유세포생장인자（bFGF）、혈관내피생장인자（VEGF）적표체급의의。방법장24지대백토분위대조조화실험조，대조조용광알밀정은（SD-Ag ），실험조용PRP。재7、10、14 d ，량조분별수궤선취4지대백토마취처사후취재，행창면유합솔비교、H E염색급면역조직화학검측。결과7、10、14 d실험조창면유합솔균교대조조고（ P＜0．05）。7 d시，실험조육아조직조현저，세포급혈관수량밀집。10 d시，실험조가견모세혈관량급성섬유세포교대조조경위봉부。14 d시，실험조현시대부분성섬유세포전화위섬유세포이모세혈관수감소。7、10 d bFGF급VEGF양성세포수량조균증고，실험조명현고우대조조（P＜0．05），이14 d bFGF급 VEGF양성세포수량조균축보하강（P＞0．05）。결론 PRP가이촉진대백토탕상창면유합，주요궤제시명현증가조기창면중bFGF급VEGF표체。
Objective To discuss the significance of platelet rich plasma in promoting bFGF and VEGF expression in would healing of rabbit buns .Methods The 24 rabbits were randomly divided into control group and experimental group .The sulfadiazine silver paint to the wound for control group and the platelet rich plasma gel evenly spread to the wound for experimental group .At 7th ,10th ,14th day ,4 rabbits of each group were randomly selected to sacrificed after anesthesiaed ,wound healing rate was compare in 2 groups ,HE staining and testimmunohistochemistry were conducted in each group .Results At 7th ,10th ,14th day ,the wound healing rate of experimental group were higher than those of control group .At 7th d ,the focal granulation ,cells and vessels inten-sive were more significant in experimental group than in control group ,at 10th day ,the wound fibroblast cells and capillary number are more significant in experimental group ,and at 14th day ,the most of fibroblasts translate into fiber cell and the capillary number decreased ,the fibroblasts proliferation was still active and fiber cell was less in the control group .At 7th ,10th day ,the expression of bFGF and VEGF were both higher ,but experimental group is obvious higher than control group(P<0 .05) ,but at 14th day ,the ex-pression of bFGF and VEGF were gradually declined and was not obvious statistics differences in the two group (P>0 .05) .Conclu-sion The platelet rich plasma could promote the big white rabbit scald wound healing ,while the main mechanism maybe the ex-pression of bFGF and VEGF increased in the early .