CAJ | 학술논문

目的：通过观察寿胎丸对复发性流产小鼠蜕膜组织α-烯醇化酶表达的影响,探讨其安胎的作用机制。方法 CBA/J×BALB/C建立正常妊娠模型,CBA/J×DBA/2建立复发性流产模型,将复发性流产模型CBA/J× DBA/2孕鼠按怀孕先后顺序随机分为模型组及寿胎丸高、中、低剂量组,从妊娠第1日开始灌胃给药,至孕14 d处死小鼠,采用免疫组化与蛋白印迹方法检测各组蜕膜组织α-烯醇化酶的表达。结果小鼠模型组与正常组比较,α-烯醇化酶表达明显升高,差异有统计学意义(P＜0.01)；与模型组比较,寿胎丸高、中、低剂量组α-烯醇化酶表达均降低,差异有统计学意义(P＜0.01)；寿胎丸高剂量组α-烯醇化酶表达与中、低剂量组之间差异无统计学意义(P＞0.05)。结论寿胎丸通过下调复发性流产小鼠蜕膜组织α-烯醇化酶的表达,从而实现维持妊娠,这可能是其安胎的作用机制之一。
목적：통과관찰수태환대복발성유산소서세막조직α-희순화매표체적영향,탐토기안태적작용궤제。방법 CBA/J×BALB/C건립정상임신모형,CBA/J×DBA/2건립복발성유산모형,장복발성유산모형CBA/J× DBA/2잉서안부잉선후순서수궤분위모형조급수태환고、중、저제량조,종임신제1일개시관위급약,지잉14 d처사소서,채용면역조화여단백인적방법검측각조세막조직α-희순화매적표체。결과소서모형조여정상조비교,α-희순화매표체명현승고,차이유통계학의의(P＜0.01)；여모형조비교,수태환고、중、저제량조α-희순화매표체균강저,차이유통계학의의(P＜0.01)；수태환고제량조α-희순화매표체여중、저제량조지간차이무통계학의의(P＞0.05)。결론수태환통과하조복발성유산소서세막조직α-희순화매적표체,종이실현유지임신,저가능시기안태적작용궤제지일。
Objective To observe the effect of Shoutaiwan on the expression ofα-enolase in the decidua tissue of recurrent abortion mice. Methods The abortion-prone CBA/J × DBA/2 matings were established as the model of recurrent abortion and the nonabortion-prone CBA/J×BALB/C matings were used as the model of normal pregnancy. The model of recurrent abortion CBA/J × DBA/2 of pregnant mice were randomly divided into model group and Shoutaiwan high-, medium-, low-dose groups, pregnant mice of every group were orally administrated in different doses. On the 14th day of pregnancy, the mice were killed. The expression ofα-enolase was detected by using immunohistochemical method and Western Blot. Results α-enolase expression in the model group was significantly higher than the normal pregnancy group (P<0.01). Compared with the model group, Shoutaiwan low-, medium- and high-dose group significantly decreasedα-enolase expression of pregnant mice (P <0.01). Compared with high-dose group, Shoutaiwan medium-and low-dose group showed no significant difference (P>0.05). Conclusion Shoutaiwan could down-regulate the expression ofα-enolase in the decidua tissues of recurrent abortion mice, which may be one of its mechanisms of preventing miscarriage.