CAJ | 학술논문

目的：研究不同纯度高良姜素对人黑色素瘤细胞-人永生化角质形成细胞(A375-HaCaT)共培养模型中黑素细胞增殖及黑素合成的影响。方法利用Transwell技术建立A375-HaCaT共培养体系,以0.1、0.5、1.0、5.0、10μg/mL纯度为70%和90%的高良姜素作用于共培养模型。采用MTT比色法检测细胞增殖,NaOH裂解法测定黑素含量,多巴氧化法测定酪氨酸酶活性,ELISA法检测HaCaT细胞因子干细胞生长因子(SCF)、内皮素(ET)-1的表达。结果 A375-HaCaT共培养模型中的A375细胞生长良好,0.1、0.5、1.0、5.0、10μg/mL纯度为70%和90%的高良姜素对共培养体系中的A375细胞增殖、酪氨酸酶活性及黑素合成均具有上调作用。各纯度高良姜素浓度≥0.5μg/mL时,能够提高HaCaT细胞中的ET-1和SCF等细胞因子的表达水平,且纯度70%的高良姜素作用强度优于90%的高良姜素。结论高良姜素能显著促进A375-HaCaT共培养模型中A375的细胞增殖及黑素合成,其作用机制可能与高良姜素上调HaCaT细胞中的ET-1和SCF等细胞因子的表达水平有关。
목적：연구불동순도고량강소대인흑색소류세포-인영생화각질형성세포(A375-HaCaT)공배양모형중흑소세포증식급흑소합성적영향。방법이용Transwell기술건립A375-HaCaT공배양체계,이0.1、0.5、1.0、5.0、10μg/mL순도위70%화90%적고량강소작용우공배양모형。채용MTT비색법검측세포증식,NaOH렬해법측정흑소함량,다파양화법측정락안산매활성,ELISA법검측HaCaT세포인자간세포생장인자(SCF)、내피소(ET)-1적표체。결과 A375-HaCaT공배양모형중적A375세포생장량호,0.1、0.5、1.0、5.0、10μg/mL순도위70%화90%적고량강소대공배양체계중적A375세포증식、락안산매활성급흑소합성균구유상조작용。각순도고량강소농도≥0.5μg/mL시,능구제고HaCaT세포중적ET-1화SCF등세포인자적표체수평,차순도70%적고량강소작용강도우우90%적고량강소。결론고량강소능현저촉진A375-HaCaT공배양모형중A375적세포증식급흑소합성,기작용궤제가능여고량강소상조HaCaT세포중적ET-1화SCF등세포인자적표체수평유관。
Objective To investigate the effect of Galangin of different purity on melanocyte proliferation and melanin synthesis of A375-HaCaT co-culture system. Methods The A375-HaCaT co-culture system was established with Transwell technology, and 0.1, 0.5, 1.0, 5.0, 10 μg/mL Galangin of the purity of 70%and 90%was used to act on the co-culture system. Cell proliferation, melanin content and tyrosinase of A375 were measured by MTT assay, NaOH lysis assay and L-DOPA oxidation assay respectively. The cytokines such as ET-1 and SCF in HaCaT were detected by ELISA. Results A375 cells in co-culture system grew well, and 0.1, 0.5, 1.0, 5.0, 10 μg/mL Galangin of the purity of 70% and 90% had up-regulation effect on cell proliferation, melanin content and tyrosinase of A375. Moreover, Galangin could increase the expression level of ET-1 and SCF of HaCaT at more than 0.5 μg/mL, and the effect of Galangin of 70% purity was better than 90% purity. Conclusion Galangin could promote the cell proliferation, melanin content and tyrosinase of A375, and mechanism of the pathways is probably related to the up-regulation on the expression of ET-1 and SCF of HaCaT.