CAJ | 학술논문

目的：比较不同工艺提取的竹节参皂苷粗提物的毒性及抗炎效果。方法采用不同工艺分别制备竹节参皂苷粗提物样品1、样品2、样品3、样品4、样品5、样品6,然后用不同工艺、不同浓度的样品处理RAW264.7细胞,显微镜下观察细胞形态,噻唑蓝(MTT)法检测细胞活力,一氧化氮(NO)试剂盒检测 RAW264.7细胞 NO 释放量。结果不同样品作用于RAW264.7细胞毒性从小到大依次为：样品4＜样品6＜样品5＜样品2＜样品1＜样品3。不同样品对脂多糖刺激的RAW264.7细胞NO释放抑制效果依次为：样品4＞样品5＞样品2＞样品6＞样品1＞样品3。结论6种不同提取工艺得到的竹节参皂苷粗提物中,泡沫分离法提取的竹节参皂苷粗提物毒性最小,抗炎效果最好。
목적：비교불동공예제취적죽절삼조감조제물적독성급항염효과。방법채용불동공예분별제비죽절삼조감조제물양품1、양품2、양품3、양품4、양품5、양품6,연후용불동공예、불동농도적양품처리RAW264.7세포,현미경하관찰세포형태,새서람(MTT)법검측세포활력,일양화담(NO)시제합검측 RAW264.7세포 NO 석방량。결과불동양품작용우RAW264.7세포독성종소도대의차위：양품4＜양품6＜양품5＜양품2＜양품1＜양품3。불동양품대지다당자격적RAW264.7세포NO석방억제효과의차위：양품4＞양품5＞양품2＞양품6＞양품1＞양품3。결론6충불동제취공예득도적죽절삼조감조제물중,포말분리법제취적죽절삼조감조제물독성최소,항염효과최호。
Objective To compare the toxicity and anti-inflammatory effect of total saponins from Panax japonicus by different extraction technology. Methods The total saponins of sample 1, sample 2, sample 3, sample 4, sample 5 and sample 6 was prepared respectively by different process, and RAW264.7 cells were treated with the samples of different concentration. Then cells morphology was observed under microscope, thiazolyl blue (MTT) method was used to detect cell activity, the nitric oxide (NO) release of RAW264.7 cells was detected with NO kit. Results The cell toxicity of different samples from low to high was as follows:sample 4<sample 6<sample 5<sample 2<sample 1<sample 3. The NO inhibitory effect on RAW264.7 cells stimulated by LPS was as follows:sample 4>sample 5>sample 2>sample 6>sample 1>sample 3. Conclusion Among these six different kinds of extraction process of total saponins from Panax japonicus, the total saponins extract by foam fractionation has not only the minimal toxicity, but also the best primary anti-inflammatory effect.