中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
1期
75-76,77
,共3页
真菌性角膜炎%墨汁-KOH湿片法%荧光染色法
真菌性角膜炎%墨汁-KOH濕片法%熒光染色法
진균성각막염%묵즙-KOH습편법%형광염색법
Fungal keratitis%Ink-KOH wet method%Fluorescent staining method
目的：探讨真菌性角膜炎快速而有效的临床实验室诊断方法。方法：对2012年6-12月53例临床疑似真菌性角膜炎患者送检的角膜刮片标本，同时进行墨汁-KOH湿片法镜检、荧光染色镜检和真菌培养。以真菌培养为诊断金标准，对墨汁-KOH湿片法与荧光染色法进行方法学比较。结果：32例真菌培养阳性标本，墨汁-KOH湿片法20例阳性，阳性率为62.5%，荧光染色28例阳性，阳性率为87.5%，两者阳性率比较差异有统计学意义（字2=6.125，P=0.025）；两种涂片镜检共为阳性的20例，共为阴性的4例。结论：荧光染色法镜检可以提高真菌性角膜炎的阳性诊断率，是真菌性角膜炎快速、简单、有效的临床实验室诊断方法。
目的：探討真菌性角膜炎快速而有效的臨床實驗室診斷方法。方法：對2012年6-12月53例臨床疑似真菌性角膜炎患者送檢的角膜颳片標本，同時進行墨汁-KOH濕片法鏡檢、熒光染色鏡檢和真菌培養。以真菌培養為診斷金標準，對墨汁-KOH濕片法與熒光染色法進行方法學比較。結果：32例真菌培養暘性標本，墨汁-KOH濕片法20例暘性，暘性率為62.5%，熒光染色28例暘性，暘性率為87.5%，兩者暘性率比較差異有統計學意義（字2=6.125，P=0.025）；兩種塗片鏡檢共為暘性的20例，共為陰性的4例。結論：熒光染色法鏡檢可以提高真菌性角膜炎的暘性診斷率，是真菌性角膜炎快速、簡單、有效的臨床實驗室診斷方法。
목적：탐토진균성각막염쾌속이유효적림상실험실진단방법。방법：대2012년6-12월53례림상의사진균성각막염환자송검적각막괄편표본，동시진행묵즙-KOH습편법경검、형광염색경검화진균배양。이진균배양위진단금표준，대묵즙-KOH습편법여형광염색법진행방법학비교。결과：32례진균배양양성표본，묵즙-KOH습편법20례양성，양성솔위62.5%，형광염색28례양성，양성솔위87.5%，량자양성솔비교차이유통계학의의（자2=6.125，P=0.025）；량충도편경검공위양성적20례，공위음성적4례。결론：형광염색법경검가이제고진균성각막염적양성진단솔，시진균성각막염쾌속、간단、유효적림상실험실진단방법。
Objective:To study the rapid and effective clinical laboratory diagnostic methods of fungal keratitis.Method:Specimens of 53 clinical suspected patients with fungal keratitis from June 2012 to December 2012 were selected,for the ink-KOH wet method microscopy,fluorescence staining microscopy and fungal culture at the same time. Fungus culture as the diagnostic gold standard,the results of ink-KOH wet method microscopy and fluorescent staining microscopy were compared.Result:In 32 cases of positive specimens of fungal culture,20 cases were positive in ink-KOH wet microscopy,the positive rate was 62.5%,28 cases were positive in fluorescent staining microscopy,the positive rate was 87.5%,Two positive rate comparisons difference was statistically significant(X2=6.125,P=6.125). There were 20 cases both kinds of smear microscopy were positive,4 cases were both negative.Conclusion:The fluorescence staining microscopy can improve the positive diagnosis rate of fungal keratitis,it is a kind of rapid, simple and effective method of clinical laboratory diagnosis.
