黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2014年
1期
977-978,979
,共3页
付璀莹%孙煌%狄斌%白政忠
付璀瑩%孫煌%狄斌%白政忠
부최형%손황%적빈%백정충
亲水作用色谱(HILIC)%盐酸多巴酚丁胺注射液%盐酸半胱氨酸
親水作用色譜(HILIC)%鹽痠多巴酚丁胺註射液%鹽痠半胱氨痠
친수작용색보(HILIC)%염산다파분정알주사액%염산반광안산
HILIC%Dobutamine Hydrochloride Injection%L-cysteine hydrochloride
目的：建立亲水作用色谱测定盐酸多巴酚丁胺注射液中盐酸半胱氨酸含量的方法。方法：采用默克ZIC-HILIC柱（150 mm×4.6 mm,5μm）；乙腈-20 mM甲酸铵溶液(80：20,pH6.2)为流动相；检测波长206 nm。结果：盐酸半胱氨酸在0.2mg/ml～2.0mg/ml范围内呈良好的线性关系，平均回收率99.3%，RSD为0.6%。结论：此方法简便、灵敏度高，重现性好，可用于盐酸多巴酚丁胺注射液中盐酸半胱氨酸的含量测定。
目的：建立親水作用色譜測定鹽痠多巴酚丁胺註射液中鹽痠半胱氨痠含量的方法。方法：採用默剋ZIC-HILIC柱（150 mm×4.6 mm,5μm）；乙腈-20 mM甲痠銨溶液(80：20,pH6.2)為流動相；檢測波長206 nm。結果：鹽痠半胱氨痠在0.2mg/ml～2.0mg/ml範圍內呈良好的線性關繫，平均迴收率99.3%，RSD為0.6%。結論：此方法簡便、靈敏度高，重現性好，可用于鹽痠多巴酚丁胺註射液中鹽痠半胱氨痠的含量測定。
목적：건립친수작용색보측정염산다파분정알주사액중염산반광안산함량적방법。방법：채용묵극ZIC-HILIC주（150 mm×4.6 mm,5μm）；을정-20 mM갑산안용액(80：20,pH6.2)위류동상；검측파장206 nm。결과：염산반광안산재0.2mg/ml～2.0mg/ml범위내정량호적선성관계，평균회수솔99.3%，RSD위0.6%。결론：차방법간편、령민도고，중현성호，가용우염산다파분정알주사액중염산반광안산적함량측정。
Objective:To estabilish a HILIC method for determination of L-cysteine hydrochloride in Dobutamine Hydrochloride In-jection. Method:The separation was performed on MERK ZIC-HILIC column(150 mm×4.6mm,5μm)with acetonitrile-20mM ammoni-um formate as mobile phase(80:20,pH6.2);the detection wavelength was 206nm. Results:There was a good liner relationship of L-cysteine hydrochloride with the range of 0.2mg/ml~2.0mg/ml.The average recovery was 99.3%(RSD=0.6%) .Conclusion:The method is simple, sensitive and reliable,it can be used as quantitative analysis method for L-cysteine hydrochloride in Dobutamine hydrochloride injection.
