CAJ | 학술논문

目的：建立可同时测定双黄连口服液中黄芩苷、连翘苷、绿原酸，3种质控成分含量的HPLC方法.方法：色谱柱为Welchrom C18柱（4.6 mm×150 mm,5μm）；流动相为甲醇(A)-0.2%磷酸(B)水溶液，梯度洗脱，分段变波长测定；柱温：30℃；体积流量1.0 mL/min。结果：3种成分的线性关系均良好，精密度、稳定性、重复性的RSD均低于2%，加样回收率为95%～120%。结论：本方法准确、灵敏、可靠，重现性较好，可用于双黄连口服液的质量控制。
목적：건립가동시측정쌍황련구복액중황금감、련교감、록원산，3충질공성분함량적HPLC방법.방법：색보주위Welchrom C18주（4.6 mm×150 mm,5μm）；류동상위갑순(A)-0.2%린산(B)수용액，제도세탈，분단변파장측정；주온：30℃；체적류량1.0 mL/min。결과：3충성분적선성관계균량호，정밀도、은정성、중복성적RSD균저우2%，가양회수솔위95%～120%。결론：본방법준학、령민、가고，중현성교호，가용우쌍황련구복액적질량공제。
Objective:Estabilish a HPLC method which can masure the quality control compontent of baicalin SHL oral liquid, phillyrin and chlorogenic acid simultaneously. Methods:Chromatographic column:welchrom C18(4.6 mm×150 mm, 5μm);The mobile phase:an aqueous solution of methanol(A)-0.2%phosphoric acid(B),gradient elution, measure by segmentation variable wavelength;col-umn temperature:30℃;volume flow rate:1.0 mL/min. Results:the linear relationship of this three ingredient were all well and the RSD of precision, stability and repeatability were all less than 2%.The average recovery was 95%-120%. Conclusion:this method is accurate, sensitive, reliable and has a good reproducibility, can be used for quality control of Shuanghuanglian oral liquid.