CAJ | 학술논문

目的：研究东莨菪碱经鼻腔给药的脑靶向性。方法将Wistar大鼠分为两组，分别通过腹腔和鼻腔给予0．3 mg东莨菪碱。用固相萃取和液质联用的方法测定在5、10、20、30、60、120、240、480 min血浆和脑组织匀浆中东莨菪碱的浓度，计算并比较腹腔和鼻腔给药后的药代动力学参数。结果东莨菪碱鼻腔给药的生物利用度为65％。经鼻腔给药后的脑药浓度大于经腹腔给药的脑药浓度，鼻腔给药东莨菪碱的脑药浓度与血药浓度的比值远大于腹腔给药后脑药浓度与血药浓度的比值（P＜0．05）。结论东莨菪碱鼻腔给药具有脑靶向性。
목적：연구동랑탕감경비강급약적뇌파향성。방법장Wistar대서분위량조，분별통과복강화비강급여0．3 mg동랑탕감。용고상췌취화액질련용적방법측정재5、10、20、30、60、120、240、480 min혈장화뇌조직균장중동랑탕감적농도，계산병비교복강화비강급약후적약대동역학삼수。결과동랑탕감비강급약적생물이용도위65％。경비강급약후적뇌약농도대우경복강급약적뇌약농도，비강급약동랑탕감적뇌약농도여혈약농도적비치원대우복강급약후뇌약농도여혈약농도적비치（P＜0．05）。결론동랑탕감비강급약구유뇌파향성。
Objective To investigate the distribution of scopolamine into brain following intranasal .Methods Adult Wistar rats were divided into two groups ,and administrated with scopolamine 0 .3 mg via intraperitoneal or intranasal respectively .Two groups of blood concentration and brain concentration of scopolamine were determined by solid phase extraction (SPE) and LC/MS assay at the time point of 5 ,10 ,20 ,30 ,60 ,120 ,240 ,480 min ,and their kinetic parameters were calculated and compared .Results Scopol-amine bioavailability of intranasal administration was 65% ,and the maximum concentration of scopolamine in brain following in-tranasal administration was much higher than that following intraperitoneal administration .Comparing the ratio of AUC in brain and in plasma ,ratio of intranasal administration is bigger than that of intraperitoneal administration significantly (P<0 .05) .Con-clusion It was concluded that scopolamine′s intranasal administration induced the agent targeting distribution into the brain .