CAJ | 학술논문

目的：对济南战区陆勤干部血管紧张素转换酶（ACE）基因多态性分布情况进行调查分析。方法采用罗氏Cy-cler480II型荧光定量PCR分析仪进行基因型判断。同时随机抽样进行琼脂糖凝胶电泳分型进行对比分析。结果济南战区陆勤干部ACE基因DD型占23．3％，ID型占43．2％，II型占33．5％，D和I等位基因频率分别为0．44和0．56；济南战区陆勤干部女性ACE基因II型频率最高，而男性ACE基因ID型频率最高，差异具有统计学意义（P＜0．05）；男性与女性等位基因频率差异无统计学意义（P＞0．05）。结论济南战区陆勤干部ACE基因多态性以II型和I型等位基因占优势；进行ACE基因调查对于早期发现和及时预防某些相关疾病有十分重要的意义。
목적：대제남전구륙근간부혈관긴장소전환매（ACE）기인다태성분포정황진행조사분석。방법채용라씨Cy-cler480II형형광정량PCR분석의진행기인형판단。동시수궤추양진행경지당응효전영분형진행대비분석。결과제남전구륙근간부ACE기인DD형점23．3％，ID형점43．2％，II형점33．5％，D화I등위기인빈솔분별위0．44화0．56；제남전구륙근간부녀성ACE기인II형빈솔최고，이남성ACE기인ID형빈솔최고，차이구유통계학의의（P＜0．05）；남성여녀성등위기인빈솔차이무통계학의의（P＞0．05）。결론제남전구륙근간부ACE기인다태성이II형화I형등위기인점우세；진행ACE기인조사대우조기발현화급시예방모사상관질병유십분중요적의의。
Objective To investigate and analysis the situation of angiotensin-converting enzyme (ACE) gene polymorphism in military service officers of Jinan theater .Methods Roche Cycler480II fluorescence quantitative PCR analyzer was used to detected ACE genotype .Meanwhile ,some samples were randomly analyzed by agarose gel electrophoresis .Results ACE DD genotype ac-counted for 23 .3% in the military service officers of Jinan theater cadres ,ID type accounted for 43 .2% ,II type accounted for 33 .5% ,D and I allele frequencies were 0 .44 and 0 .56 ,respectively ;type II ACE gene frequency was highest in female cadres ,while frequency of ID type was highest in male cadres ,the difference was statistically significant between two groups (P< 0 .05);the difference of allele frequency between men and women was not statistically significant (P>0 .05) .Conclusion ACE gene polymor-phism with type II and Iallele were dominant in the military service officers of Jinan theater cadres ;ACE gene polymorphism survey is important for early detection and timely prevention of certain related diseases .