CAJ | 학술논문

目的：探讨过表达高迁移率族蛋白A2（HMGA2）对人垂体生长激素腺瘤细胞增殖及周期的影响。方法 HMGA2过表达质粒转染原代培养人垂体生长激素腺瘤细胞（转染组）。蛋白免疫印迹法（Western blot）、细胞增殖实验（cell counting kit-8，CCK-8）、流式细胞仪术分别检测 HMGA2过表达质粒转染垂体生长激素腺瘤细胞后对 HMGA2蛋白表达、细胞增殖、细胞周期的影响。结果24 h后转染组垂体生长激素腺瘤细胞HMGA2蛋白表达水平高于空载体组（转染空质粒的细胞）（P＜0．05）；较空载体组在24、48、72、96 h 4个时间点OD值较大，差异均有统计学意义（P＜0．05）；转染组细胞增殖数量增加，与空载体组相比转染组细胞G1期所占比例减少，S期所占比例增加，差异有统计学意义（P＜0．05）。结论过表达 HMGA2促进垂体腺瘤细胞增殖，促进细胞G1期进展。
목적：탐토과표체고천이솔족단백A2（HMGA2）대인수체생장격소선류세포증식급주기적영향。방법 HMGA2과표체질립전염원대배양인수체생장격소선류세포（전염조）。단백면역인적법（Western blot）、세포증식실험（cell counting kit-8，CCK-8）、류식세포의술분별검측 HMGA2과표체질립전염수체생장격소선류세포후대 HMGA2단백표체、세포증식、세포주기적영향。결과24 h후전염조수체생장격소선류세포HMGA2단백표체수평고우공재체조（전염공질립적세포）（P＜0．05）；교공재체조재24、48、72、96 h 4개시간점OD치교대，차이균유통계학의의（P＜0．05）；전염조세포증식수량증가，여공재체조상비전염조세포G1기소점비례감소，S기소점비례증가，차이유통계학의의（P＜0．05）。결론과표체 HMGA2촉진수체선류세포증식，촉진세포G1기진전。
Objective To explore the effects of over-expression high-mobility-group A2(HMGA2) on proliferation and cell cycle in primary cultured human growth hormone-secreting pituitary tumor cells .Methods The protein expression of HMGA2 in growth hormone-secreting pituitary tumor cells transfected by HMGA2 overexpression plasmid (transfection group) were detected by Western blot .The effects of the HMGA2 overexpression on cell proliferation ,cell cycle were measured by (cell counting kit-8 , CCK-8) assay and flow cytometry respectively .Results HMGA2 protein of transfection group was higher than the blank load transfection group(tranfectied with pcDNA3 .1 plasmid) after 24 h (P<0 .05) .To compare with the blank load transfection group , cells overexpression of HMGA2 could drastically increase the ability of proliferation at 24 ,48 ,72 ,96 h and increase the S phase rati-o of cell cycle and decrease the G1 phase ratio of cell cycle ,the difference was statistically significant (P<0 .05) .Conclusion Over-expression of HMGA2 increases pituitary tumor cells proliferation ability and promote cells G 1 phase progress .