CAJ | 학술논문

目的：观察颈内动脉移植骨髓间充质干细胞（BMSCs）透过血脑屏障（BBB）在血管性痴呆（VD）大鼠脑内存活、迁移及对认知能力的影响。方法分离大鼠股骨骨髓单核细胞，以贴壁培养法培养BMSCs，分化诱导并作BrdU标记；选72只Wistar大鼠分为对照组、模型组、治疗组3组，制作VD大鼠模型，治疗组于术后24h颈内动脉移植0．5mL1．2×107/mL的BrdU标记细胞。移植4、8周后检测移植细胞在VD大鼠脑内存活、迁移以及大鼠学习记忆能力。结果颈内动脉移植后骨髓间充质干细胞广泛存活于VD大鼠脑组织内，随着时间的延长，迁移方向集中，主要聚集至海马、大脑皮质等缺血易损伤区域。移植后4、8周，模型组大鼠主动回避反应（AAR）比率[（42．1±4．5）、（43．6±3．6）]低于对照组[（90．0±4．3）、（92．5±5．0）]，差异有统计学意义（P＜0．01）；治疗组（69．2±4．7，70．8±4．7）较模型组明显提高，差异有统计学意义（P＜0．01）。结论骨髓间充质干细胞颈内动脉移植后可透过BBB向脑缺血易损伤部位迁移聚集、存活，促进缺颈内动脉移植后骨血损伤后脑组织的修复与再生，明显增强VD大鼠学习记忆能力。
목적：관찰경내동맥이식골수간충질간세포（BMSCs）투과혈뇌병장（BBB）재혈관성치태（VD）대서뇌내존활、천이급대인지능력적영향。방법분리대서고골골수단핵세포，이첩벽배양법배양BMSCs，분화유도병작BrdU표기；선72지Wistar대서분위대조조、모형조、치료조3조，제작VD대서모형，치료조우술후24h경내동맥이식0．5mL1．2×107/mL적BrdU표기세포。이식4、8주후검측이식세포재VD대서뇌내존활、천이이급대서학습기억능력。결과경내동맥이식후골수간충질간세포엄범존활우VD대서뇌조직내，수착시간적연장，천이방향집중，주요취집지해마、대뇌피질등결혈역손상구역。이식후4、8주，모형조대서주동회피반응（AAR）비솔[（42．1±4．5）、（43．6±3．6）]저우대조조[（90．0±4．3）、（92．5±5．0）]，차이유통계학의의（P＜0．01）；치료조（69．2±4．7，70．8±4．7）교모형조명현제고，차이유통계학의의（P＜0．01）。결론골수간충질간세포경내동맥이식후가투과BBB향뇌결혈역손상부위천이취집、존활，촉진결경내동맥이식후골혈손상후뇌조직적수복여재생，명현증강VD대서학습기억능력。
Objective To observe the survival and migration characteristics after intracarotid transplantation of bone marrow stem cells(BMSCs) and its effect on learning-memory abilities across the blood-brain barrier(BBB) in the vascular dementia(VD) rats .Methods Bone mononuclear cells(BMNCs) were isolated from bone marrow in vitro by standard Ficoll-Hypaque technique , then cells were enriched and expanded by using bone marrow adherent culture .72 Wistar rats were meanly divided into control group ,model group and treatment group .The VD rat model was established by modified pulsinellis 4-vessel occlusion(4 VO) .The treatment group received intracarotid infusion of 0 .5 mL 1 .2 × 107/mL BMSCs which were labeled with BrdU in vitro after opera-tion .Their survival ,migration and the learning-memory abilities were observed at 4th and 8th week .Results BMSCs transplanted by intracarotid transplantation survived and had been found throughout the brain tissue .They major migrated and localized in the is-chemic zone of injury such as hippocampus and cerebral cortex .Compared with the control group ,active avoidance response(AAR) ratio in the model group(42 .1 ± 4 .5) ,group(43 .6 ± 3 .6)showed significantly decrease compared with the control group (90 .0 ± 4 .3) ,(92 .5 ± 5 .0)(P<0 .01) ,and the treatment group(69 .2 ± 4 .7) ,(70 .8 ± 4 .7)was significant higher than the model group(P<0 .01) .Conclusion Intracarotid transplantation of BMSCs could enter the VD rats cerebra parenchyma via BBB ,migrate into the damaged brain tissue to gather and survive .Learning-memory abilities can be improved significantly by transplanted BMSCs .