华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
z1期
400-402
,共3页
温立斌%何孔旺%解建平%杨浩%周建新%金全胜%王玉然
溫立斌%何孔旺%解建平%楊浩%週建新%金全勝%王玉然
온립빈%하공왕%해건평%양호%주건신%금전성%왕옥연
类猪圆环病毒因子P1%形态学%免疫电镜技术
類豬圓環病毒因子P1%形態學%免疫電鏡技術
류저원배병독인자P1%형태학%면역전경기술
Porcine circovirus-like agent P1%Virus morphology%Immunoelectron microscopy
采用氯化铯平衡密度梯度离心获得较为纯净的病毒粒子后，经过免疫电镜技术（液相免疫电镜法和免疫胶体金标记法）对P1病毒进行了观察鉴定。结果表明，P1病毒呈球形、无囊膜、直径约为25 nm。
採用氯化銫平衡密度梯度離心穫得較為純淨的病毒粒子後，經過免疫電鏡技術（液相免疫電鏡法和免疫膠體金標記法）對P1病毒進行瞭觀察鑒定。結果錶明，P1病毒呈毬形、無囊膜、直徑約為25 nm。
채용록화색평형밀도제도리심획득교위순정적병독입자후，경과면역전경기술（액상면역전경법화면역효체금표기법）대P1병독진행료관찰감정。결과표명，P1병독정구형、무낭막、직경약위25 nm。
The results of porcine circovirus-like agent P1 morphology were reported here .Relatively pure viral particles were obtained by CsCl equilibrium density-gradient centrifugation ,and then detected by immune electron microscopy ( Both liquid phase immune electron microscopy and immunogold labeling electron microscopy ) .The re-sults showed that P1 is spherical in shape ,non-enveloped and about 25 nm in diameter.
