华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
z1期
397-399
,共3页
温立斌%何孔旺%解建平%杨浩%周建新%金全胜%王玉然
溫立斌%何孔旺%解建平%楊浩%週建新%金全勝%王玉然
온립빈%하공왕%해건평%양호%주건신%금전성%왕옥연
类猪圆环病毒因子P1%纯化%免疫亲和层析
類豬圓環病毒因子P1%純化%免疫親和層析
류저원배병독인자P1%순화%면역친화층석
Porcine circovirus-like agent P1%Purification%Immuno-affinity chromatography
通过免疫亲和层析对类猪圆环病毒因子P1纯化进行了研究。采用环氧活化及NHS活化的琼脂糖凝胶，与兔抗P1表位肽抗体偶联，对P1进行了免疫亲和吸附，通过电镜技术证实获得了纯化病毒。结果表明，免疫亲和层析是有效的P1病毒纯化方法。
通過免疫親和層析對類豬圓環病毒因子P1純化進行瞭研究。採用環氧活化及NHS活化的瓊脂糖凝膠，與兔抗P1錶位肽抗體偶聯，對P1進行瞭免疫親和吸附，通過電鏡技術證實穫得瞭純化病毒。結果錶明，免疫親和層析是有效的P1病毒純化方法。
통과면역친화층석대류저원배병독인자P1순화진행료연구。채용배양활화급NHS활화적경지당응효，여토항P1표위태항체우련，대P1진행료면역친화흡부，통과전경기술증실획득료순화병독。결과표명，면역친화층석시유효적P1병독순화방법。
This study deals mainly with porcine circovirus-like agnet P1 antigen purified by immuno-affinity chromatography .The purified P1 virus, which came from elution buffers from adsorption column coupled anti-P1 epitope-containing peptide antibody to either Epoxy-activated agarose or NHS-activated agarose , was confirmed by transmission electron microscopic observations .The study suggests that purified P 1 antigen by immuno-affinity chro-matography is an effective way .
