CAJ | 학술논문

采用叶面喷施水杨酸、病原菌培养滤液、草酸等因子诱导花生植株对网斑病抗病性，并测定寄主防御酶活性变化和NPR1基因表达量，来探讨花生诱导抗病性及作用机制。结果表明：诱导处理可以使花生获得系统抗病性，且以2．0 mmol/L水杨酸诱导花生网斑病抗性效果最好，控制病害效果达到35．3％；寄主防御酶活性升高与诱导抗病性强度呈正相关，但几种防御酶在诱导抗病性应答反应中的变化存在显著差异；实时荧光定量 PCR 分析表明：2．0 mmol/L的SA诱导处理后花生叶片中 NPR1表达量明显升高，与诱导抗病性密切相关。
채용협면분시수양산、병원균배양려액、초산등인자유도화생식주대망반병항병성，병측정기주방어매활성변화화NPR1기인표체량，래탐토화생유도항병성급작용궤제。결과표명：유도처리가이사화생획득계통항병성，차이2．0 mmol/L수양산유도화생망반병항성효과최호，공제병해효과체도35．3％；기주방어매활성승고여유도항병성강도정정상관，단궤충방어매재유도항병성응답반응중적변화존재현저차이；실시형광정량 PCR 분석표명：2．0 mmol/L적SA유도처리후화생협편중 NPR1표체량명현승고，여유도항병성밀절상관。
Induce peanut plants to spot disease resistance by spraying salicylic acid on leaves ,pathogen culture filtrate,oxalic acid and other factors .Then determine the changes of host defense enzyme activity and NPR1 gene expression to explore the induced disease resistance and its mechanism .Results show that peanut can get systemic resistance by induction .Best results for 2 .0 mmol/L salicylic acid induced peanuts and the controlling efficacy was 35 .3%.The host defense enzyme activity was positively correlated with the intensity of induced disease resistance , but the induced disease resistance response changes of several defense enzymes varies greatly from the other .Real-time fluorescent quantitative PCR analysis showed that the tendency for 2 .0 mmol/L of SA induced NPR1 expres-sion in peanut leaf volume increased significantly after treatment ,which is closely related to the induction of disease resistance .