CAJ | 학술논문

旨在研究不同物种泌乳奶牛牛乳中活性物质免疫球蛋白和乳铁蛋白绝对含量的特性。采用双抗夹心ELISA法对牦牛乳、水牛乳和荷斯坦奶牛乳乳样中主要免疫球蛋白（IgA、IgG、IgM）和乳铁蛋白（Lf）含量进行了定量测定并做出了相关性分析。结果显示，牦牛乳、水牛乳和荷斯坦奶牛乳乳样中免疫球蛋白和乳铁蛋白活性质量浓度与吸光值存在良好的线性关系，各标准曲线方程的R均大于0．99。不同物种牛乳中IgA、IgG、IgM和Lf的绝对含量差异显著（P ＜0．05）。同一品种牛乳中免疫球蛋白IgA、IgG、IgM和Lf的绝对含量显示出不同的个体性差异。除荷斯坦奶牛乳乳样中IgA与IgM呈现较低程度的负相关外，牛乳中IgA、IgG、IgM和Lf在同一物种内均呈现出不同程度的正相关趋势。结果表明，不同物种牛乳中免疫球蛋白和乳铁蛋白的绝对含量受遗传因素的影响较大且其含量各具特点，根据同一品种牛乳中IgA、IgG、IgM和Lf含量的相关性可对其含量进行相互预测。
지재연구불동물충비유내우우유중활성물질면역구단백화유철단백절대함량적특성。채용쌍항협심ELISA법대모우유、수우유화하사탄내우유유양중주요면역구단백（IgA、IgG、IgM）화유철단백（Lf）함량진행료정량측정병주출료상관성분석。결과현시，모우유、수우유화하사탄내우유유양중면역구단백화유철단백활성질량농도여흡광치존재량호적선성관계，각표준곡선방정적R균대우0．99。불동물충우유중IgA、IgG、IgM화Lf적절대함량차이현저（P ＜0．05）。동일품충우유중면역구단백IgA、IgG、IgM화Lf적절대함량현시출불동적개체성차이。제하사탄내우유유양중IgA여IgM정현교저정도적부상관외，우유중IgA、IgG、IgM화Lf재동일물충내균정현출불동정도적정상관추세。결과표명，불동물충우유중면역구단백화유철단백적절대함량수유전인소적영향교대차기함량각구특점，근거동일품충우유중IgA、IgG、IgM화Lf함량적상관성가대기함량진행상호예측。
The objective of the current study was to characterize the concentrations of immunoglobulins ( IgA, IgG,IgM) and lactoferrin in different bovine milk.Sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantify the immunoglobulins and lactoferrin (Lf) contents in Yak milk,Buffalo milk and Holstein cow milk sam-ples,and correlation analysis was completed .The results showed that immunoglobulins and lactoferrin in Yak milk , Buffalo milk and Holstein cow milk samples had a good linear relationsship between concentration and absorbance values,and the standard curve equation R was greater than 0.99.There were significant differences of IgA ,IgG, IgM,and Lf in different milk(P<0.05).The contents of IgA,IgG,IgM and Lf of species showed remarkable indi-vidual differences .IgA and IgM in Holstein cow milk showed a negative correlation ,while the others species had a positive correlation .The results indicated that the content of immunoglobulins and lactoferrin in the milk were influ-enced by genetic factors and had each characteristic .According to the correlation of immunoglobulins and lacto-ferrin,forecasting the content of immunoglobulins and lactoferrin within species may be possible .