CAJ | 학술논문

利用29对多态性较好的微卫星引物对哲罗鱼保种群体332个个体（由4个子代群体组成）进行遗传多样性的分析。结果表明：29对引物扩增的等位基因数为2～13，平均有效等位基因数为3．9613，平均观测杂合度Ho和平均期望杂合度He分别为0．642和0．6751，平均多态信息含量PIC为0．633。4个子代群体的遗传多样性趋势为B7（F1♀×野生♂3×3）略高于B10（F1♀×F1♂3×3），B10（F1♀×F1♂3×3）略高于B6（野生♀×野生♂3×3）， B9（ F1♀×F1♂1×1）最低。4个群体间的遗传距离趋势为：除F1♀×F1♂1×1群体外，野生♀×野生♂3×3群体与F1♀×F1♂3×3群体之间的遗传距离最远，F1♀×野生♂3×3群体与F1♀×F1♂3×3群体之间遗传距离最近。基于个体间遗传距离构建的聚类树结果表明，自繁群体B6（野生♀×野生♂3×3）、B9（F1♀×F1♂1×1）、B10（F1♀×F1♂3×3）各自聚在一起，杂交群体B7（F1♀×野生♂3×3）则分为2支。本研究讨论了种群数量较少的珍稀动物保持后代遗传多样性丰富的可能性。
이용29대다태성교호적미위성인물대철라어보충군체332개개체（유4개자대군체조성）진행유전다양성적분석。결과표명：29대인물확증적등위기인수위2～13，평균유효등위기인수위3．9613，평균관측잡합도Ho화평균기망잡합도He분별위0．642화0．6751，평균다태신식함량PIC위0．633。4개자대군체적유전다양성추세위B7（F1♀×야생♂3×3）략고우B10（F1♀×F1♂3×3），B10（F1♀×F1♂3×3）략고우B6（야생♀×야생♂3×3）， B9（ F1♀×F1♂1×1）최저。4개군체간적유전거리추세위：제F1♀×F1♂1×1군체외，야생♀×야생♂3×3군체여F1♀×F1♂3×3군체지간적유전거리최원，F1♀×야생♂3×3군체여F1♀×F1♂3×3군체지간유전거리최근。기우개체간유전거리구건적취류수결과표명，자번군체B6（야생♀×야생♂3×3）、B9（F1♀×F1♂1×1）、B10（F1♀×F1♂3×3）각자취재일기，잡교군체B7（F1♀×야생♂3×3）칙분위2지。본연구토론료충군수량교소적진희동물보지후대유전다양성봉부적가능성。
The genetic diversity of the taimen conservation population ( Contained 4 groups ) with 29 polymor-phic microsatellite loci .The study showed that in the 332 sampled individuals ,2-13 alleles were detected in each locus,the average effective allele number was 3.961 3,the average observed heterozygosity and expected heterozy-gosity were 0.642 and 0.675 1 respectively,the average PIC was 0.633.The genetic diversity of the 4 groups was B7(F1♀×wild♂3 ×3) exceed B10(F1♀×F1♂3 ×3),B10(F1♀×F1♂3 ×3) exceed B6(wild♀×wild♂3 ×3) and B9(F1♀×F1♂1 ×1) was the lowest.Except the group B9(F1♀×F1♂1 ×1),the genetic distance between B6(wild♀×wild♂3 ×3) and B10(F1♀×F1♂3 ×3) was the farthest,between B7(F1♀×wild♂3 × 3 ) and B10 ( F1♀×F1♂3 ×3 ) with the nearest distance .The clustering result based on the genetic distance showed that B6(wild♀×wild♂3 ×3),B9(F1♀×F1♂1 ×1),B10(F1♀×F1♂3 ×3) clustered as one clade respectively ,the cross breeding groups B 7 ( F1♀×wild♂3 ×3 ) was divided into two clades .This research also discussed the possibility that the endangered animals to maintain the rich diversity in offsprings .