CAJ | 학술논문

利用磁珠富集法分离草金鱼的微卫星分子序列，获得38个阳性克隆，对其进行测序，获得31个微卫星DNA序列，利用软件Primer Premier 5．0设计引物12对，其中有8对引物可扩增出稳定的目的条带。利用筛选出的8对微卫星引物对北京2个草金鱼养殖群体进行了遗传多样性分析。结果表明，2个草金鱼养殖群体的遗传多样性水平较高，其平均观测杂合度（Ho）分别为0．5655，0．5343，平均期望杂合度（He）分别为0．6570，0．6286，平均多态性信息含量PIC分别为0．5106，0．5009。两群体间遗传距离为0．0957，遗传相似度为0．9211。8个微卫星位点可用于金鱼群体的遗传学研究。
이용자주부집법분리초금어적미위성분자서렬，획득38개양성극륭，대기진행측서，획득31개미위성DNA서렬，이용연건Primer Premier 5．0설계인물12대，기중유8대인물가확증출은정적목적조대。이용사선출적8대미위성인물대북경2개초금어양식군체진행료유전다양성분석。결과표명，2개초금어양식군체적유전다양성수평교고，기평균관측잡합도（Ho）분별위0．5655，0．5343，평균기망잡합도（He）분별위0．6570，0．6286，평균다태성신식함량PIC분별위0．5106，0．5009。량군체간유전거리위0．0957，유전상사도위0．9211。8개미위성위점가용우금어군체적유전학연구。
Microsatellite DNA was isolated from goldfish with combining biotin capture method .We obtained 38 positive clones and isolated 31 microsatellite sequences .12 pairs of primers were designed by Primer Premier 5 .0 and 8 pairs of primers can obtain amplifying products among them .The genetic diversity of the artificial breed gold-fish populations were studied by using the 8 Microsatellite primers .The results showed that the genetic diversity lev-el of two populations were high.The average heterozygosity(Ho) in two populations was from 0.534 3 to 0.565 5, average excepted heterozygosity (He) was from 0.628 6 to 0.657 0,and average polymorphism information content (PIC) was from 0.500 9 to 0.510 6.The genetic distance was 0.095 7 and genetic identity was 0.921 1.All of 8 microsatellite locus could be used for genetic analysis in goldfish .