CAJ | 학술논문

烟草青枯病、黑胫病和猝倒病是烟草生产中的重要病害，常发生复合侵染。以3种重要烟草病原菌基因组DNA作为目的基因，分别设计3对特异性引物，通过优化引物和模板浓度，摸索扩增参数，建立了能同时检测3种病害的多重聚合酶链式反应（ Multiplex polymerase chain reaction ，mPCR）体系。结果表明，多重PCR能分别扩增出长度为461，364，265 bp的特异性目的条带，能同时检测3种病原菌DNA的最低检出限为1．01 ng/μL，该多重PCR检测体系反应准确、快速、高效，为同时检测以上3种烟草病害提供了新的方法，对烟草复合侵染病害的诊断和防治提供了重要的指导作用。
연초청고병、흑경병화졸도병시연초생산중적중요병해，상발생복합침염。이3충중요연초병원균기인조DNA작위목적기인，분별설계3대특이성인물，통과우화인물화모판농도，모색확증삼수，건립료능동시검측3충병해적다중취합매련식반응（ Multiplex polymerase chain reaction ，mPCR）체계。결과표명，다중PCR능분별확증출장도위461，364，265 bp적특이성목적조대，능동시검측3충병원균DNA적최저검출한위1．01 ng/μL，해다중PCR검측체계반응준학、쾌속、고효，위동시검측이상3충연초병해제공료신적방법，대연초복합침염병해적진단화방치제공료중요적지도작용。
R.solanacearum,P.nicotianae and P.aphanidermatum are important compound infection diseases of the tobacco production .In this study , Three pairs of primers were designed respectively according to the genomic DNA of R.solanacearum,P.nicotiana and P.aphanidermatum.And they were used to amplify simultaneously the DNA sequences by multiplex PCR and the reaction conditions were optimized .The results showed that the multiplex PCR assay amplified three specific target channels of 461 ,364 and 265 bp.The detection limits of the multiplex PCR for R.solanacearum,P.nicotiana and P.aphanidermatum were 1.01 ng/μL.This multiplex PCR method was accurate ,fast and effective and provided a new approach and an important guiding role for the tobacco disease com -pound infection diagnosis and treatment .