CAJ | 학술논문

采用微卫星标记技术，对吉富品系尼罗罗非鱼（ GIFT strain）抗病群体和易感病群体进行了遗传差异分析。结果显示：在30个微卫星位点中20个能扩增出多态性高、重复性好的清晰条带；抗病群体和易感病群体的平均等位基因数分别为2．5，2．4个；观测杂合度和期望杂合度分别为0．469和0．441，0．440和0．476；多态信息含量PIC分别为0．359和0．379；UNH845和UNH189位点在抗病群体中偏离Hardy-Weinberg平衡（P＜0．05），GM180位点在易感病群体偏离Hardy-Weinberg平衡，2个群体间的遗传距离和遗传分化指数为0．055和0．054。此外，GM251和GM462位点的差异等位基因片段（135，198 bp）在抗病群体中出现的频率显著高于易感病群体，推测这2个基因片段与抗病性状存在一定的连锁关系。以上结果表明，吉富品系尼罗罗非鱼抗病群体与易感病群体之间的遗传多样性差异不明显，筛选出2个与抗病性状相关的微卫星标记，为开展吉富品系尼罗罗非鱼抗病品种分子标记辅助选育奠定了基础。
채용미위성표기기술，대길부품계니라라비어（ GIFT strain）항병군체화역감병군체진행료유전차이분석。결과현시：재30개미위성위점중20개능확증출다태성고、중복성호적청석조대；항병군체화역감병군체적평균등위기인수분별위2．5，2．4개；관측잡합도화기망잡합도분별위0．469화0．441，0．440화0．476；다태신식함량PIC분별위0．359화0．379；UNH845화UNH189위점재항병군체중편리Hardy-Weinberg평형（P＜0．05），GM180위점재역감병군체편리Hardy-Weinberg평형，2개군체간적유전거리화유전분화지수위0．055화0．054。차외，GM251화GM462위점적차이등위기인편단（135，198 bp）재항병군체중출현적빈솔현저고우역감병군체，추측저2개기인편단여항병성상존재일정적련쇄관계。이상결과표명，길부품계니라라비어항병군체여역감병군체지간적유전다양성차이불명현，사선출2개여항병성상상관적미위성표기，위개전길부품계니라라비어항병품충분자표기보조선육전정료기출。
Technology with microsatellite was used to analyze the genetic difference between disease -resistant and disease-susceptible groups of GIFT strain .The results show that there were 20 loci successfully amplified with stable target bands within 30 tested loci to identify the polymorphism for the tested GIFT strain .The average alleles in disease-resistant was 2.5 slightly higher than that of 2.4 in disease-susceptible group .Then the average of ob-served heterozygosity(Ho)and expected heterozygosity(He)were 0.469 and 0.441,0.440 and 0.476 respectively, and the polymorphism information content (PIC)was 0.359 and 0.379.Genetic similarity index and genetic distance was 0.055 and 0.054 respectively.The locus UNH845,UNH189 and GM180 showed significant departure from Hardy-Weinberg equilibrium after sequential bonferroni correction (P<0.05)in two groups.GM251 and GM462 sites differences of allele fragment (135,198 bp) were positively related to disease-resistance GIFT strain.Above mentioned results indicated that the genetic diversity between disease-resistant and disease-susceptible group of GIFT strain was not significantly different ,and the GM251 and GM462 gene fragment with its differential bands from the PCR ampliftcation can be used as the molecular marker for the disease-resistant GIFT strain molecular assistant selection .