华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
z1期
7-11
,共5页
乔燕春%林锦英%谢伟平%张华%郑岩松%谢丽芳%李莲芳%黄红弟
喬燕春%林錦英%謝偉平%張華%鄭巖鬆%謝麗芳%李蓮芳%黃紅弟
교연춘%림금영%사위평%장화%정암송%사려방%리련방%황홍제
华南地区%瓜类蔬菜%SRAP%遗传相关性
華南地區%瓜類蔬菜%SRAP%遺傳相關性
화남지구%과류소채%SRAP%유전상관성
South China%Gourd vegetable%SRAP%Genetic relation
利用9个表型性状和20对SRAP标记分析了13份华南地区主要瓜类作物的遗传进化关系,9个表型性状差异显著,20对SRAP分子标记分析供试的13份材料,共产生328条差异谱带,仅有2条共同带,多态性比率为99%,遗传相似系数为0.140~0.993。进一步分析了表型性状与SRAP分子标记的相关性,P=0.05时,相关系数为0.576;说明表型性状和分子标记结果存在一定的显著相关,通过分子标记研究和表型性状的相关性分析,确定华南地区主要葫芦科植物的遗传关系,可为今后在分子水平进行葫芦科瓜类种质资源的育种及亲缘关系分析等研究提供科学依据。
利用9箇錶型性狀和20對SRAP標記分析瞭13份華南地區主要瓜類作物的遺傳進化關繫,9箇錶型性狀差異顯著,20對SRAP分子標記分析供試的13份材料,共產生328條差異譜帶,僅有2條共同帶,多態性比率為99%,遺傳相似繫數為0.140~0.993。進一步分析瞭錶型性狀與SRAP分子標記的相關性,P=0.05時,相關繫數為0.576;說明錶型性狀和分子標記結果存在一定的顯著相關,通過分子標記研究和錶型性狀的相關性分析,確定華南地區主要葫蘆科植物的遺傳關繫,可為今後在分子水平進行葫蘆科瓜類種質資源的育種及親緣關繫分析等研究提供科學依據。
이용9개표형성상화20대SRAP표기분석료13빈화남지구주요과류작물적유전진화관계,9개표형성상차이현저,20대SRAP분자표기분석공시적13빈재료,공산생328조차이보대,부유2조공동대,다태성비솔위99%,유전상사계수위0.140~0.993。진일보분석료표형성상여SRAP분자표기적상관성,P=0.05시,상관계수위0.576;설명표형성상화분자표기결과존재일정적현저상관,통과분자표기연구화표형성상적상관성분석,학정화남지구주요호호과식물적유전관계,가위금후재분자수평진행호호과과류충질자원적육충급친연관계분석등연구제공과학의거。
The genetic diversity of 13 main gourd vegetable in South China were analyzed using 9 morphological characters and SRAP markers with 20 pairs of primers .The results showed that there were the significantly different in 9 morphological characters .A total of 328 polymorphism fragments and 2 common fragments of 13 main gourd vegetables were generated from 20 pairs of SRAP primers .The polymorphism rate reached 99%,the genetic similar-ity values were between 0.140-0.993.The correlation coefficient was 0.576 (P =0.05,this study reveals) of the phenotypic traits and SRAP markers , the result revealed that there were the significantly relation between the phenotypic traits and SRAP markers .The correlation analysis could help our clarify the genetic relation of main gourd vegetable in South China ,and provide the scientic basis for the study of genetic relation and breeding of gourd vegetable in the Cucurbitacea .