CAJ | 학술논문

利用28对SSR核心引物构建了25个鲁棉研系列抗虫棉品种的分子指纹图谱并进行遗传多样性分析。28对核心引物的SSR位点分属21条染色体，等位基因数为2～8个，平均4．39个；PIC值为0．63～0．93，平均0．84。数字指纹图谱显示，最少采用BNL1421、BNL1513、BNL1231、NAU2026和BNL2646这5对引物组合可以将25份供试材料区分开。对其中14个常规品种的遗传多样性分析表明，其遗传相似系数介于0．35～0．87，平均为0．57。此结果可为现阶段鲁棉研系列种质资源的杂交选育提供依据。
이용28대SSR핵심인물구건료25개로면연계렬항충면품충적분자지문도보병진행유전다양성분석。28대핵심인물적SSR위점분속21조염색체，등위기인수위2～8개，평균4．39개；PIC치위0．63～0．93，평균0．84。수자지문도보현시，최소채용BNL1421、BNL1513、BNL1231、NAU2026화BNL2646저5대인물조합가이장25빈공시재료구분개。대기중14개상규품충적유전다양성분석표명，기유전상사계수개우0．35～0．87，평균위0．57。차결과가위현계단로면연계렬충질자원적잡교선육제공의거。
SSR markers were used to analyze the fingerprinting and genetic diversity of 25 SCRC series transgenic Bt cotton varieties.Twenty-eight primers screened out from 412 SSR primers,with high polymorphisms andgood repeatability,were regarded as core primers in the study.These primers whose SSR loci distributed on 21 chromosomesof cotton,have 2 -8 alleles,with an average of 4.39;polymorphic information content(PIC)about 0.63 -0.93,with an average of 0.84.These results suggest the core primers have preferable polymorphism .The digital fingerprintingindicated all varieties could be distinguished by 5 primer combinations at least.UPGMA cluster analysiswas used to estimate the genetic distances and to construct a dendrogram by NTSYS 2.1.The similarity coefficientsof 14 SCRC conventional varieties were between 0.35 -0.87,with an average of 0.57,indicating relative abundanceof genetic diversity.These results can provide technical support for cross breeding of SCRC germplasm re -sources.