郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
1期
25-28
,共4页
罗毅鑫%黄祺%唐春宇%程学敏%高亚琳%陈虹钖%黄楠%李桉琪%薛玉堂%左其亭%巴月%崔留欣
囉毅鑫%黃祺%唐春宇%程學敏%高亞琳%陳虹钖%黃楠%李桉琪%薛玉堂%左其亭%巴月%崔留訢
라의흠%황기%당춘우%정학민%고아림%진홍양%황남%리안기%설옥당%좌기정%파월%최류흔
铅%儿童%生长激素%生长激素结合蛋白%胰岛素样生长因子-1%胰岛素样生长因子结合蛋白3
鉛%兒童%生長激素%生長激素結閤蛋白%胰島素樣生長因子-1%胰島素樣生長因子結閤蛋白3
연%인동%생장격소%생장격소결합단백%이도소양생장인자-1%이도소양생장인자결합단백3
lead%children%growth hormone%growth hormone binding protein%insulin-like growth factor-1%insulin-like growth factor binding protein 3
目的：探讨沙颍河铅污染现状及其对儿童生长激素-胰岛素样生长因子-1（ GH-IGF-1）轴和体格发育的影响。方法：随机选择淮河沙颍河流域S县两个村庄作为调查点（依距河岸距离远近分别定为对照区和污染区）；原子吸收分光光度法检测河水和调查点饮用水及土壤中的铅含量。整群抽取两村庄小学8～13岁在校学生作为研究对象，伏安极谱法测定儿童血清铅含量；ELISA法检测儿童血清胰岛素样生长因子-1（ IGF-1）、胰岛素样生长因子结合蛋白3（IGFBP3）、生长激素（GH）和生长激素结合蛋白（GHBP）水平；电子体重计、身高坐高计、皮褶厚度计及Gulick卷尺分别测量体重、身高、皮褶厚度和胸围。结果：河水3个采样断面铅浓度均超过国家地表水水质标准Ⅴ级。污染区饮用水及土壤铅含量均高于对照区（ t＝2．663和2．300，P＜0．05）。污染区儿童血清铅含量高于对照区儿童（t＝3．227，P＝0．002）。污染区男孩身高、体重、皮褶厚度、胸围与对照区比较差异均无统计学意义（P均＞0．05）。两区女孩身高、体重和胸围差异均无统计学意义（P均＞0．05），但污染区女孩皮褶厚度高于对照区（t＝3．036，P＝0．003）。结论：沙颍河流域的饮用水中铅含量明显升高，污染区儿童血清GH-IGF-1轴相关因子水平受到影响。
目的：探討沙潁河鉛汙染現狀及其對兒童生長激素-胰島素樣生長因子-1（ GH-IGF-1）軸和體格髮育的影響。方法：隨機選擇淮河沙潁河流域S縣兩箇村莊作為調查點（依距河岸距離遠近分彆定為對照區和汙染區）；原子吸收分光光度法檢測河水和調查點飲用水及土壤中的鉛含量。整群抽取兩村莊小學8～13歲在校學生作為研究對象，伏安極譜法測定兒童血清鉛含量；ELISA法檢測兒童血清胰島素樣生長因子-1（ IGF-1）、胰島素樣生長因子結閤蛋白3（IGFBP3）、生長激素（GH）和生長激素結閤蛋白（GHBP）水平；電子體重計、身高坐高計、皮褶厚度計及Gulick捲呎分彆測量體重、身高、皮褶厚度和胸圍。結果：河水3箇採樣斷麵鉛濃度均超過國傢地錶水水質標準Ⅴ級。汙染區飲用水及土壤鉛含量均高于對照區（ t＝2．663和2．300，P＜0．05）。汙染區兒童血清鉛含量高于對照區兒童（t＝3．227，P＝0．002）。汙染區男孩身高、體重、皮褶厚度、胸圍與對照區比較差異均無統計學意義（P均＞0．05）。兩區女孩身高、體重和胸圍差異均無統計學意義（P均＞0．05），但汙染區女孩皮褶厚度高于對照區（t＝3．036，P＝0．003）。結論：沙潁河流域的飲用水中鉛含量明顯升高，汙染區兒童血清GH-IGF-1軸相關因子水平受到影響。
목적：탐토사영하연오염현상급기대인동생장격소-이도소양생장인자-1（ GH-IGF-1）축화체격발육적영향。방법：수궤선택회하사영하류역S현량개촌장작위조사점（의거하안거리원근분별정위대조구화오염구）；원자흡수분광광도법검측하수화조사점음용수급토양중적연함량。정군추취량촌장소학8～13세재교학생작위연구대상，복안겁보법측정인동혈청연함량；ELISA법검측인동혈청이도소양생장인자-1（ IGF-1）、이도소양생장인자결합단백3（IGFBP3）、생장격소（GH）화생장격소결합단백（GHBP）수평；전자체중계、신고좌고계、피습후도계급Gulick권척분별측량체중、신고、피습후도화흉위。결과：하수3개채양단면연농도균초과국가지표수수질표준Ⅴ급。오염구음용수급토양연함량균고우대조구（ t＝2．663화2．300，P＜0．05）。오염구인동혈청연함량고우대조구인동（t＝3．227，P＝0．002）。오염구남해신고、체중、피습후도、흉위여대조구비교차이균무통계학의의（P균＞0．05）。량구녀해신고、체중화흉위차이균무통계학의의（P균＞0．05），단오염구녀해피습후도고우대조구（t＝3．036，P＝0．003）。결론：사영하류역적음용수중연함량명현승고，오염구인동혈청GH-IGF-1축상관인자수평수도영향。
Aim:To investigate the effect of lead from Shaying River on children's growth hormone , insulin-like growth factor-1 (GH-IGF-1) axis and physical development .Methods:Two villages in S county were selected randomly as pollu-ted area and control area according to the distance from the Shaying River Basin .Lead levels were measured both in the riv-er water and the drinking water .Children aged 8 to 13 years studying in the village primary school were recruited by cluster sampling.The concentration of lead in serum was measured by polarographic method .Serum insulin-like growth factor-1 (IGF-1), insulin-like growth factor binding protein 3 (IGFBP3), growth hormone (GH), and growth hormone binding proteins (GHBP) were measured by ELISA.The indexes including body weight , height,skinfold thickness and chest-circ-umference were measured .Results:The concentrations of lead exceeded classⅤstandards of the surface water quality in section of the river .Compared with control area , the concentration of lead both in drinking water and soil was significantly higher than those in the polluted area (t=2.663 and 2.300,P<0.05).Serum lead level in children from the polluted are-a was higher than that from control area (t=3.227,P=0.002).Height, weight, skinfold thickness and chest-circumfer-ence did not show significant differences between the boys of the two groups (P>0.05).Only skinfold thickness had sig-nificant difference between the girls of the two groups (t=3.036,P=0.003).Conclusion:The concentration of lead in drinking water of the Shaying River Basin has increased significantly .The GH-IGF-1 axis of children in the polluted areas has been affected .
