CAJ | 학술논문

目的：：探讨胰腺癌组织中 Drosha 蛋白表达状况及其临床意义。方法：收集胰腺癌样本85例及对应癌旁组织53例，采用 H&E和免疫组化法检测胰腺癌组织标本中 Drosha 蛋白的表达并分析其与临床病理因素之间的关系。采用定量PCR方法检测Drosha基因表达。结果：胰腺癌组织中 Drosha 的表达阳性率为72.9%(62/85),而相应癌旁 Drosha表达阳性率为100%（53/53），两者表达差异有统计学意义（P=0.000),Drosha 蛋白在胰腺癌中的阳性强度平均积分值由10.3分降至2.3分。Drosha蛋白的表达与肿瘤的分期(c2=19.2, P=0.0003)、分级(c2=10.8, P=0.013)、淋巴结转移(c2=23.6, P=0.00003)等密切相关。与对应癌旁组织相比，Drosha基因在胰腺癌组织中表达下调（P=0.000）。结论： Drosha蛋白在胰腺癌组织中表达下降，Drosha 可能在胰腺癌的发生、发展中具有重要作用。
목적：：탐토이선암조직중 Drosha 단백표체상황급기림상의의。방법：수집이선암양본85례급대응암방조직53례，채용 H&E화면역조화법검측이선암조직표본중 Drosha 단백적표체병분석기여림상병리인소지간적관계。채용정량PCR방법검측Drosha기인표체。결과：이선암조직중 Drosha 적표체양성솔위72.9%(62/85),이상응암방 Drosha표체양성솔위100%（53/53），량자표체차이유통계학의의（P=0.000),Drosha 단백재이선암중적양성강도평균적분치유10.3분강지2.3분。Drosha단백적표체여종류적분기(c2=19.2, P=0.0003)、분급(c2=10.8, P=0.013)、림파결전이(c2=23.6, P=0.00003)등밀절상관。여대응암방조직상비，Drosha기인재이선암조직중표체하조（P=0.000）。결론： Drosha단백재이선암조직중표체하강，Drosha 가능재이선암적발생、발전중구유중요작용。
Objective :To investigate the expression of Drosha and its clinicopathological significance in pancreatic cancer tis-sues. Methods H&E staining and immunohistochemical staining technology were used to detect the expression of Drosha, and its cor-relation with clinicopathological characteristics was studied. And the RT-PCR was performed to detect the gene expression level of Drosha . Results The positive rates of Drosha were 76.5%(65/85) in pancreatic cancer tissues and 100%(53/53) in adjacent non-can-cer tissues, and the difference was statistically significant(P<0.05); the expression of Drosha had a significantly positive correlation with the tumor stage( Chi-Square=19.2, P=0.0003), grade (Chi-Square=10.8, P=0.013) and lymph node metastasis (Chi-Square=23.6, P = 0.00003). The gene expression of Drosha was down-regulated in these paired 53 pancreatic cancer patients compared to those adjacent pancreatic tissues (P=0.000) . Conclusion Drosha is low expressed in pancreatic cancer tissues, and may play impor-tant roles in pancreatic cancer biogenesis and progress.