CAJ | 학술논문

目的通过体外实验研究观察稳定转染hTNF-α/293基因细胞对人结肠癌( LOVO)细胞生长增殖影响。方法采用RT-PCR和ELISA检测稳定转染hTNF-α/293基因细胞和Hek-293细胞mRNA和蛋白表达水平。观察体外培养中,hTNF-α/293基因细胞与LOVO细胞共同培养,于不同的时间点,采用噻唑蓝MTT法检测LOVO肿瘤细胞增殖的活性。结果重组质粒转染Hek-293细胞后,Western blot检测到了hTNFα蛋白体外表达,检测表明hTNFα/293细胞组和hTNFα阳性组对共培养的LOVO细胞的增殖具有明显的抑制作用,且具有良好的量效关系。结论提示稳定转染hTNF-α/293对LOVO细胞增殖有明显抑制效应,且呈现出良好的数量依赖关系。由于TNF-α/293基因细胞可有效分泌hTNFα蛋白,并能分泌到细胞外。
목적통과체외실험연구관찰은정전염hTNF-α/293기인세포대인결장암( LOVO)세포생장증식영향。방법채용RT-PCR화ELISA검측은정전염hTNF-α/293기인세포화Hek-293세포mRNA화단백표체수평。관찰체외배양중,hTNF-α/293기인세포여LOVO세포공동배양,우불동적시간점,채용새서람MTT법검측LOVO종류세포증식적활성。결과중조질립전염Hek-293세포후,Western blot검측도료hTNFα단백체외표체,검측표명hTNFα/293세포조화hTNFα양성조대공배양적LOVO세포적증식구유명현적억제작용,차구유량호적량효관계。결론제시은정전염hTNF-α/293대LOVO세포증식유명현억제효응,차정현출량호적수량의뢰관계。유우TNF-α/293기인세포가유효분비hTNFα단백,병능분비도세포외。
objective To observe the effects of co-culture of hTNFα-sercreting and human colon cancer cells LOVO on the proliferation of cancer cells. Methods The stable transfected hTNF-α/293 , mRNA of Hek-293 cell and protein expression were detected by RT-PCR and ELISA. The positive group was added hTNF-αfactor,and MTT assay was applied under the optical density 490 nm. Through human tumor cell proliferation inhibition experiment,the inhibitory effects on colon cancer cells ( LOVO) proliferation were observed. Results hTNF-α/293 cells and hTNF-α-positive group showed a significant lower A,which suggested that hTNF-α/293 cells and hTNF-α-positive group had significant inhibition on the proliferation of colon cancer cells. Conclusion The inhibition of hTNF-αsecreted by hTNF-α/293 cells on co-lon cancer cell proliferation shows significant dose-effect dependency,and hTNF-αexpresses a considerable inhibition on the colon cancer cell proliferation as positive drug.