CAJ | 학술논문

目的：研究姜黄素对原代培养的肺巨噬细胞表达TLR4mRNA及分泌TNF-a、IL-6和IL-8的影响。方法用脂多糖（LPS）建立体外肺巨噬细胞体外炎症损伤模型，用ELISA观察肺巨噬细胞分泌TNF-a、IL-6和IL-8和用RT-PCR、western blot观察肺巨噬细胞TLR4的表达。结果 LPS明显诱导肺巨噬细胞分泌TNF-a、IL-6和IL-8增加及TLR4的增量表达（P<0.01），姜黄素可抑制这种作用（P<0.05），且与姜黄素药物浓度有关（P>0.05）。结论姜黄素对肺巨噬细胞的抗炎机制可能是通过抑制TLR4的表达，通过脂多糖-TLR4信号传导通路，从而减少炎性细胞因子的释放；姜黄素有可能作为一种有研究潜力的抗感染治疗的中药。
목적：연구강황소대원대배양적폐거서세포표체TLR4mRNA급분비TNF-a、IL-6화IL-8적영향。방법용지다당（LPS）건입체외폐거서세포체외염증손상모형，용ELISA관찰폐거서세포분비TNF-a、IL-6화IL-8화용RT-PCR、western blot관찰폐거서세포TLR4적표체。결과 LPS명현유도폐거서세포분비TNF-a、IL-6화IL-8증가급TLR4적증량표체（P<0.01），강황소가억제저충작용（P<0.05），차여강황소약물농도유관（P>0.05）。결론강황소대폐거서세포적항염궤제가능시통과억제TLR4적표체，통과지다당-TLR4신호전도통로，종이감소염성세포인자적석방；강황소유가능작위일충유연구잠력적항감염치료적중약。
Objective To study the influence of Curcumin on the expressions of TLR4 and TNF-a、IL-6 、IL-8 release in pulmonary macrophage. Methods The experiments were performed in culture of LPS induced pulmonary macrophage in vitro,the TNF-a、IL-6 、IL-8 release was detected with ELISA and the expressions of TLR4 was detected with RT-PCR and western blot. Results Pulmonary macrophage was stimulated with LPS and the expressions of TLR4 and TNF-a 、IL-6、IL-8 release was increased (P<0.01),but it was inhibited by Curcumin (P<0.05),and dose dependence (P<0.05). Conclusion The mechanism of Curcumin’s antiinflammatory may be reduce cytokine release,by inhibits expressions of TLR4 and via Cross talk toll-like receptor-4 signaling;Curcumin may be used as a kind of anti-infective drug.