CAJ | 학술논문

目的：乳腺肿瘤细胞的转移是乳腺癌致死的主要原因。淋巴转移作为血道和淋巴两大转移途径之一，在乳腺癌转移过程中至关重要。针对乳腺癌转移的特点，本研究组设计了具有淋巴富集特性的纳米载药系统，活体动态监测其对原位乳腺肿瘤生长以及肺脏和淋巴器官转移的抑制效果，旨在为临床用药提供科学依据。方法：采用活体自发光成像实时动态地监测了聚乙二醇化磷脂（PEG-PE）包载的长春瑞滨纳米胶束抗乳腺癌体内转移的过程。结果：与未包载的游离长春瑞滨（Free Vin）相比， PEG-PE包载的长春瑞滨胶束（NanoVin）增强了长春瑞滨的抗原位瘤活性，显著抑制了乳腺肿瘤细胞的淋巴及肺脏转移。结论：通过静脉给药达到了血道转移和淋巴转移两条途径的双重清扫，为靶向药物设计提供了新思路。活体光学成像技术可动态监测肿瘤的转移，为临床应用影像技术检测药物疗效提供必要的技术手段。
목적：유선종류세포적전이시유선암치사적주요원인。림파전이작위혈도화림파량대전이도경지일，재유선암전이과정중지관중요。침대유선암전이적특점，본연구조설계료구유림파부집특성적납미재약계통，활체동태감측기대원위유선종류생장이급폐장화림파기관전이적억제효과，지재위림상용약제공과학의거。방법：채용활체자발광성상실시동태지감측료취을이순화린지（PEG-PE）포재적장춘서빈납미효속항유선암체내전이적과정。결과：여미포재적유리장춘서빈（Free Vin）상비， PEG-PE포재적장춘서빈효속（NanoVin）증강료장춘서빈적항원위류활성，현저억제료유선종류세포적림파급폐장전이。결론：통과정맥급약체도료혈도전이화림파전이량조도경적쌍중청소，위파향약물설계제공료신사로。활체광학성상기술가동태감측종류적전이，위림상응용영상기술검측약물료효제공필요적기술수단。
Objective:Breast cancer metastasis is a major cause of death. Lymphatic metastasis is one of the two main metastatic ways that is crucial to the metastasis process of breast cancer. To treat breast cancer metastasis, we prepared micelle-based drug carrier for lymphatic delivery. Methods:We used in vivo bioluminescence imaging for real-time dynamic monitoring of the inhibitory effect of polyethylene glycol phospholipid micelle encapsulating vinorelbine on breast cancer metastasis. Results: Compared with the free vinorelbine, vinorelbine-encapsulated micelles (NanoVin) showed a significantly enhanced anti-tumor activity both in primary and met-astatic tumors. Conclusion:Intravenous administration of NanoVin markedly prevented the metastasis of tumor cells through both he-matogenous and lymphatic systems. This study provides a new approach for treatment of metastatic tumors. Bioluminescence imaging is a powerful tool to dynamically monitor tumor metastasis and clinical therapeutic effect of anticancer drugs.