CAJ | 학술논문

目的：研究温石棉及其代用纤维对鼠细胞钙激活蛋白43（calciumactinated protein 43，CAP43）及B淋巴细胞瘤-2（B-cell lymghoma，Bcl-2）表达的影响，探讨温石棉及其代用品的致癌性机制。方法将不同浓度的温石棉及其代用品[四川新康温石棉（A）、陕西陕南温石棉（B）、玻璃纤维（C）、陶瓷纤维（D）、硅灰石（E）、岩棉（F），阴性对照组（G）]暴露于V79细胞，使用免疫组织化学法检测CAP43及Bcl-2的表达，并使用SPSS 21.0对表达结果进行对比分析。结果 A、B组的仓鼠肺细胞中CAP43及Bcl-2表达增强较为显著，与其他组比较，差异有统计学意义（ P＜0.05）；CAP 43及Bcl-2基因表达随着粉体浓度的增加，其OD值也呈上升趋势。结论温石棉及其代用纤维对于鼠细胞CAP43及Bcl-2表达有显著的增强作用。
목적：연구온석면급기대용섬유대서세포개격활단백43（calciumactinated protein 43，CAP43）급B림파세포류-2（B-cell lymghoma，Bcl-2）표체적영향，탐토온석면급기대용품적치암성궤제。방법장불동농도적온석면급기대용품[사천신강온석면（A）、협서협남온석면（B）、파리섬유（C）、도자섬유（D）、규회석（E）、암면（F），음성대조조（G）]폭로우V79세포，사용면역조직화학법검측CAP43급Bcl-2적표체，병사용SPSS 21.0대표체결과진행대비분석。결과 A、B조적창서폐세포중CAP43급Bcl-2표체증강교위현저，여기타조비교，차이유통계학의의（ P＜0.05）；CAP 43급Bcl-2기인표체수착분체농도적증가，기OD치야정상승추세。결론온석면급기대용섬유대우서세포CAP43급Bcl-2표체유현저적증강작용。
Objective To investigate the effect of chrysotile and its substitute on expressions of CAP 43 and Bcl-2 in hamster cells and to explore the their possible carcinogenic mechanism .Methods Chinese hamster lung cells(V79 cells)were exposed to dif-ferent concentrations of chrysotile and its substitutes including chrysotile from Xinkang ,Sichuan(A),chrysotile from Southern Shanxi (B),glass fiber(C),ceramic fiber(D),wollastonite(E)and rockwool(F).At the same time,cells without treatment were used as nega-tive controls.The expressions of CAP43 and Bcl-2 in V79 cells were detected by using immunohistochemistry ,and compared by using statistical program SPSS21.0.Results The expressions of CAP43 and Bcl-2 in A and B were significantly up-regulated(P <0.05). As the concentration of powder increased ,immune staining OD value of CAP 43 and Bcl-2 were increased .Conclusion Chrysotile and its substitute can significantly influence the CAP 43 and Bcl-2 expressions in hamster cells .