CAJ | 학술논문

目的：观察白藜芦醇对血管性痴呆大鼠的疗效及其对氧化应激作用。方法：80只健康Wistar大鼠（月龄为12～14月），体重300～400g，随机分为正常对照组、模型组、白藜芦醇对照组及白藜芦醇治疗组，每组20只。采用持久双侧颈总动脉结扎术造成血管性痴呆大鼠模型，白藜芦醇对照组及白藜芦醇治疗组于术后8周开始以白藜芦醇（25mg.kg-1）每天灌胃，正常对照组和模型组以同等量的0.5g/L羧甲基纤维素钠灌胃，连续4周。采用Morris水迷宫衡量大鼠学习记忆水平；测定大鼠脑皮层、海马组织丙二醛（MDA）、超氧化物歧化酶（SOD）及谷胱甘肽（GSH）活性的变化。结果：术后12周，与对照组相比，模型组大鼠的学习记忆能力明显下降（P<0.05）；脑皮层、海马组织内MDA的活性明显升高（P<0.05），SOD及GSH的活性明显降低（P<0.05）；与模型组相比，白藜芦醇治疗组大鼠的学习记忆能力明显提高（P<0.05）；脑皮层、海马组织内MDA的活性明显降低（P<0.05）、SOD及GSH的活性明显升高（P<0.05）。结论：白藜芦醇对血管性痴呆大鼠的学习记忆能力有明显的提高，其作用机制是通过调节脑组织内自由基损伤来实现的，该实验研究为临床上血管性痴呆的治疗提供实验基础及理论依据。
목적：관찰백려호순대혈관성치태대서적료효급기대양화응격작용。방법：80지건강Wistar대서（월령위12～14월），체중300～400g，수궤분위정상대조조、모형조、백려호순대조조급백려호순치료조，매조20지。채용지구쌍측경총동맥결찰술조성혈관성치태대서모형，백려호순대조조급백려호순치료조우술후8주개시이백려호순（25mg.kg-1）매천관위，정상대조조화모형조이동등량적0.5g/L최갑기섬유소납관위，련속4주。채용Morris수미궁형량대서학습기억수평；측정대서뇌피층、해마조직병이철（MDA）、초양화물기화매（SOD）급곡광감태（GSH）활성적변화。결과：술후12주，여대조조상비，모형조대서적학습기억능력명현하강（P<0.05）；뇌피층、해마조직내MDA적활성명현승고（P<0.05），SOD급GSH적활성명현강저（P<0.05）；여모형조상비，백려호순치료조대서적학습기억능력명현제고（P<0.05）；뇌피층、해마조직내MDA적활성명현강저（P<0.05）、SOD급GSH적활성명현승고（P<0.05）。결론：백려호순대혈관성치태대서적학습기억능력유명현적제고，기작용궤제시통과조절뇌조직내자유기손상래실현적，해실험연구위림상상혈관성치태적치료제공실험기출급이론의거。
Objective:To investigate the effects of Resveratrol on memory impairment and oxidative stress in vascular dementia (VaD) rat model. Methods:The permanent bilateral occlusion of common carotid arteries (BCCAO) rat model was used as VaD model;The Morris water maze was used to test the spatial learning and memory performance. The MDA levels,SOD activity and GSH levels in the cortex and hippocampus were measured respectively. Results:The MWM test shown that the model group rats have significantly memory impairment as compared with the control group (P<0.05). In the Cortex and Hippocampus of the model group rats the increased MDA levels were increased (P<0.05) and the SOD activity and GSH levels all decreased as compared with the control group (P<0.05). Resveratrol treatment (25mg/kg oral) can inhibit the memory impairment,the increased of MDA levels and the decreased of SOD activity and GSH levels in VaD rat model (P<0.05). Conclusion:These results confirmed the neuroprotective effects Resveratrol on VaD,and that provide novel insight into the neuroprotective effects of Resveratrol and its possible therapeutic role in VaD.