CAJ | 학술논문

目的：通过系统地评价葡萄糖氧化酶双试剂终点法不同参数设置，寻求终点法最佳参数设置模式。方法比较葡萄糖氧化酶终点法5种模式对4个不同浓度样品的分析结果，评价不同参数限制所起作用的实际表现。结果终点法5种模式中模式1（有效的线性和吸光度限制）能够对不同浓度样品进行准确地分析测量，并能对高值标本的底物耗尽现象进行有效地识别判断，其余4种模式均存在一些缺陷。结论葡萄糖氧化酶双试剂终点法在岛津CL-8000全自动生化分析仪上应用时要重视参数模式的正确选择和运用，尤其是终点吸光度A值的最高检测限额要设置在1300～1400 mAbs ，线性范围设置在24．5 mmol/L左右，才能与实际情况相符。
목적：통과계통지평개포도당양화매쌍시제종점법불동삼수설치，심구종점법최가삼수설치모식。방법비교포도당양화매종점법5충모식대4개불동농도양품적분석결과，평개불동삼수한제소기작용적실제표현。결과종점법5충모식중모식1（유효적선성화흡광도한제）능구대불동농도양품진행준학지분석측량，병능대고치표본적저물모진현상진행유효지식별판단，기여4충모식균존재일사결함。결론포도당양화매쌍시제종점법재도진CL-8000전자동생화분석의상응용시요중시삼수모식적정학선택화운용，우기시종점흡광도A치적최고검측한액요설치재1300～1400 mAbs ，선성범위설치재24．5 mmol/L좌우，재능여실제정황상부。
Objective To find out the optimal parameter setting mode by analyzing the different parameter set-tings of glucose oxidase double reagent endpoint method .Methods To compare the analytical results of 5 modes of glucose oxidase double reagent endpoint method on 4 different concentrations of samples for evaluating the real per-formance of effect of different parameter limits .Results Among 5 modes ,the first mode(effective linearity and ab-sorbance limit) could accurately analyze and measure the different concentrations of sample ,effectively identify and judge the the sample substrate depletion phenomenon of the high value samples .Some defects existed in the other 4 modes .Conclusion Should pay attention to the correct choice and use of the parameter modes of glucose oxidase double reagent endpoint method by using the Shimadzu CL-8000 fully automatic biochemical analyzer .Especially the highest detection limit of endpoint absorbance A value should be set at 1300-1400 mAbs ,the linear range should be set at 24 .5mmol/L ,which could conform to the real situation .