CAJ | 학술논문

目的：观察人乳腺癌新辅助化疗后干/祖细胞的生存及克隆形成情况。方法应用非黏附性乳腺癌细胞微球（M S ）悬浮培养法获取乳腺癌干/祖细胞；通过连续的单克隆实验检测乳腺癌细胞球细胞的克隆形成和自我更新能力，血清诱导检测其分化能力；裸鼠模型检测MS细胞移植瘤生长能力。结果化疗后乳腺癌组织内存留丰富的细胞集落，与非黏附性乳腺癌细胞微球一样，可在无血清培养基中连续传代、自我更新，能经血清诱导多向分化，还能在裸鼠体内形成与原发瘤性质相同的移植瘤，表现出干细胞样特性。结论少次化疗后乳腺癌分化细胞可被有效杀灭，但干/祖细胞仍部分存留，甚至富集，并在组织内形成乳腺癌细胞微球。
목적：관찰인유선암신보조화료후간/조세포적생존급극륭형성정황。방법응용비점부성유선암세포미구（M S ）현부배양법획취유선암간/조세포；통과련속적단극륭실험검측유선암세포구세포적극륭형성화자아경신능력，혈청유도검측기분화능력；라서모형검측MS세포이식류생장능력。결과화료후유선암조직내존류봉부적세포집락，여비점부성유선암세포미구일양，가재무혈청배양기중련속전대、자아경신，능경혈청유도다향분화，환능재라서체내형성여원발류성질상동적이식류，표현출간세포양특성。결론소차화료후유선암분화세포가피유효살멸，단간/조세포잉부분존류，심지부집，병재조직내형성유선암세포미구。
Objective To observe the stem/progenitor cells survival and the clones formation situation in hu-man breast cancer after neoadjuvant chemotherapy .Methods Human breast cancer stem/progenitor cells were en-riched by the nonadherent mammospheres(MS) suspension culture ;the serial monoclonal test was performed to de-termine the clones formation and the self-renewal ability of breast cancer stem cells ,and the differentiation ability was detected by the serum induction ;the growth ability of MS cell xenograft tumors was detected by the nude mouse model .Results Rich cell clones survived in breast cancer tissues after neoadjuvant chemotherapy ,as same as the non-adherent MS ,which had continuous passage ,self-renewal ,multi-directional differentiation ,forming xenograft tumor with the charcter similar to primary tumor and manifested the stemcell-like characteristics .Conclusion Breast cancer differentiation cells could be effectively killed after less times chemotherpy ,but the partial stem/progenitor cells still survive ,even enrich and form breast cancer MS .