CAJ | 학술논문

目的：验证筛选出的具有特异性的血清蛋白标志物，为精神分裂症寻找客观的临床诊断依据。方法利用酶联免疫吸附试验（ELISA ）技术和自动酶标仪分别测出血清特征多肽在精神疾病组和健康对照组中的吸光度值，再通过SPSS17．0对数据进行单因素方差分析，以此验证血清多肽的特异性。结果在单因素方差分析中，神经肽2在精神分裂症组与健康对照组比较，显著性水平为0．040，其他精神疾病对照组与健康对照组比较，显著性水平为0．008，说明神经肽2在这两组比较中差异有统计学意义（ P＜0．05）；在多独立样本检验中，神经肽2的渐近显著性水平为0．031，神经肽2在各组之间的表达差异有统计学意义（ P＜0．05）。结论神经肽2在精神分裂症患者与健康人之间存在明显特异性，对该病诊断、治疗和基因的研究具有重要意义。
목적：험증사선출적구유특이성적혈청단백표지물，위정신분렬증심조객관적림상진단의거。방법이용매련면역흡부시험（ELISA ）기술화자동매표의분별측출혈청특정다태재정신질병조화건강대조조중적흡광도치，재통과SPSS17．0대수거진행단인소방차분석，이차험증혈청다태적특이성。결과재단인소방차분석중，신경태2재정신분렬증조여건강대조조비교，현저성수평위0．040，기타정신질병대조조여건강대조조비교，현저성수평위0．008，설명신경태2재저량조비교중차이유통계학의의（ P＜0．05）；재다독립양본검험중，신경태2적점근현저성수평위0．031，신경태2재각조지간적표체차이유통계학의의（ P＜0．05）。결론신경태2재정신분렬증환자여건강인지간존재명현특이성，대해병진단、치료화기인적연구구유중요의의。
Objective To verify the serum protein markers with the specificity for seeking the objective clinical diagnosis basis of schizophrenia .Methods The ELISA technique and the automatic enzyme standard instrument were adopted to measure the absorbance values of serum characteristic polypeptides in the mental disease group and the health control group ,then the data were performed the one-way anova by SPSS17 .0 in order to verify the specificity of the serum polypeptides .Results By the one-way anova ,neuropeptide-2 had the significance level of 0 .040 in the comparison between the mental disease group and the healthy control group ,the significance level was 0 .008 in the comparison between the anxiety group and the healthy control group ,which indicated that neuropeptide-2 had signifi-cant difference in the comparison of these two groups ;in the more independent sample test ,the neuropeptide-2 as-ymptotic significance level was 0 .031 ,the expression of neuropeptides-2 had significant difference between the groups .Conclusion Neuropeptide 2 has obvious specificity between schizophrenia patients and normal people ,which possesses the important significance to the diagnosis ,treatment and gene research of this disease .