CAJ | 학술논문

目的探讨胃癌细胞SGC7901在体外血管生成拟态(VM)的形成过程中,自噬特异性基因Beclin-1的表达情况及其对VM形成的影响.方法分别将ShRNA整合质粒载体和空白质粒载体转染到胃癌细胞SGC7901中,采用RT-PCR及Western blot法检测SGC7901细胞中Beclin-1表达情况;并在体外构建VM模型,观察不同转染细胞中VM形成能力的差异,同时检测VM形成前后细胞中VM形成基因Notch-1的表达情况.结果胃癌SGC7901形成VM过程中,Beclin-1及Notch-1 mRNA和蛋白表达均明显升高(均P＜0.05).与转染空白质粒的SGC7901细胞相比,转染ShRNA整合质粒抑制Beclin-1后,VM形成的细胞管状结构的数目[(15.4±1.1)个比(37.8±1.9)个,P＜0.05]、长度[(316.8±24.6) mm比(385.1±14.2) mm,P＜0.05]和交点数[(11.6±1.1)个比(27.2±1.1)个,P＜0.05]明显减少,同时Notch-1表达亦明显下降(P＜0.05).结论 Beclin-1通过维持胃癌细胞VM调节基因的稳定表达促使VM形成,抑制Beclin-1为胃癌的基因治疗提供了一个可能的新靶点.
목적 탐토위암세포SGC7901재체외혈관생성의태(VM)적형성과정중,자서특이성기인Beclin-1적표체정황급기대VM형성적영향.방법 분별장ShRNA정합질립재체화공백질립재체전염도위암세포SGC7901중,채용RT-PCR급Western blot법검측SGC7901세포중Beclin-1표체정황;병재체외구건VM모형,관찰불동전염세포중VM형성능력적차이,동시검측VM형성전후세포중VM형성기인Notch-1적표체정황.결과 위암SGC7901형성VM과정중,Beclin-1급Notch-1 mRNA화단백표체균명현승고(균P＜0.05).여전염공백질립적SGC7901세포상비,전염ShRNA정합질립억제Beclin-1후,VM형성적세포관상결구적수목[(15.4±1.1)개비(37.8±1.9)개,P＜0.05]、장도[(316.8±24.6) mm비(385.1±14.2) mm,P＜0.05]화교점수[(11.6±1.1)개비(27.2±1.1)개,P＜0.05]명현감소,동시Notch-1표체역명현하강(P＜0.05).결론 Beclin-1통과유지위암세포VM조절기인적은정표체촉사VM형성,억제Beclin-1위위암적기인치료제공료일개가능적신파점.
Objective To explore the effect and mechanism of autophagy specific gene Beclin-1 in gastric cancer cell SGC7901 on vasculogenic mimicry (VM) forming ability.Methods Plasmid vectors with and without integrated shRNA were transfected respectively into SGC7901 cell line (Beclin1-inhibited group and negative control group).Simple SGC7901 cell line was used as blank group.RT-PCR and Western blot were performed to examine the expression of Beclin-1 in 3 groups.Culture was used to construct the VM model in vitro.Different VM forming ability was measured and genes (beclin-1,notch-1) expression of each group was detected before and after VM formation.Results Beclin-1 and notch-1 expression increased significantly in the process of VM forming.When beclin-1 was inhibited,the formation of VM was limited and VM formative genes expression decreased.As compared to cells of negative control group,those of Beclin1-inhibited group had less number of VM forming cellular tube-like construction (15.4±1.1 vs.37.8±1.9,P＜0.05),shorter length of such construction [(316.8±24.6) mm vs.(385.1±14.2) mm,P＜0.05],and less crossing point (11.6±1.1 vs.27.2±1.1,P＜0.05).Conclusions Beclin-1 can promote VM formation through maintaining stable expression of gastric cancer cell VM regulating genes.Beclin-1 inhibition may be a new target for gastric cancer gene therapy.