CAJ | 학술논문

对中华鳖腹腔注射浓度为0.15 mg· g-1体重的恩诺沙星，并在注射前、注射后1 h，2 h，6 h，12 h，24 h，2 d，5 d和10 d等8个时间点取样观察，研究恩诺沙星对中华鳖肝脏Ⅰ相，Ⅱ相酶活性的影响。结果表明：恩诺沙星注射后10 d内对I相的氨基比林N-脱甲基酶（ APND）、红霉素N-脱甲基酶（ ERND）以及7-乙氧基异吩唑酮-脱乙基酶（ EROD）活性均有显著抑制作用。随着注射后时间的延长，APND活性先上升后下降，最低值出现在注射后10 d；ERND活性呈显著下降趋势，到注射后24 h抑制率显著升高，之后保持稳定；恩诺沙星对EROD活性在前12 h的抑制作用要明显强于12 h之后时间段。Ⅱ相代谢酶谷胱甘肽S转移酶（ GSTs）及丙二醛（ MDA）均受恩诺沙星的显著诱导，其最高值分别出现在注射后2 h和注射后5 d。试验认为，中华鳖Ⅰ相和Ⅱ相酶均会对药物做出反应，可作为其养殖过程中药物代谢及适用性的指示物。
대중화별복강주사농도위0.15 mg· g-1체중적은낙사성，병재주사전、주사후1 h，2 h，6 h，12 h，24 h，2 d，5 d화10 d등8개시간점취양관찰，연구은낙사성대중화별간장Ⅰ상，Ⅱ상매활성적영향。결과표명：은낙사성주사후10 d내대I상적안기비림N-탈갑기매（ APND）、홍매소N-탈갑기매（ ERND）이급7-을양기이분서동-탈을기매（ EROD）활성균유현저억제작용。수착주사후시간적연장，APND활성선상승후하강，최저치출현재주사후10 d；ERND활성정현저하강추세，도주사후24 h억제솔현저승고，지후보지은정；은낙사성대EROD활성재전12 h적억제작용요명현강우12 h지후시간단。Ⅱ상대사매곡광감태S전이매（ GSTs）급병이철（ MDA）균수은낙사성적현저유도，기최고치분별출현재주사후2 h화주사후5 d。시험인위，중화별Ⅰ상화Ⅱ상매균회대약물주출반응，가작위기양식과정중약물대사급괄용성적지시물。
The effects of enrofloxacin injection (0.15mg· g-1 ) on the phaseⅠand phase Ⅱenzyme activities in liver of Chinese soft-shelled turtles , Pelodiscus sinensis were investigated in the present study .The sampling interval time were chosen at the initial stages, and 1 h, 2 h, 6 h, 12 h, 24 h, 2 d, 5 d and 10 d after injection, respectively. The results showed that the phaseⅠenzymes APND, ERND and EROD activities were all inhibited by enrofloxacin throughout the trial .The APND activity tended to recover firstly and then decreased significantly , the lowest value was observed in 10 d.ERND activity showed a decline tendency , the inhibition ratio increased significantly at 24 h and then became stable thereafter .The inhibition effect on EROD activity was much stronger at first 12 h.The GSTs activity and MDA content were both induced after enrofloxacin injection , the maximum level occurred at 2 h and 5 d, respectively.In conclusion, the present study indicated that the hepatic phaseⅠandⅡ enzyme of P.sinensis were sensitive parameters to use as biomarkers for evaluating the exposure to aquatic drugs .