CAJ | 학술논문

为进一步阐述仔猪腹泻发生的分子机制，应用生物信息学方法构建了MUC13基因参与的仔猪腹泻调控网络图谱，并对其中的基因进行基因功能聚类分析。首先，选择物种为猪，通过STRING 数据库分析发现与MUC13相关联的有10种蛋白（MUC4，MUC20，LRCH3，FLJ33471，ACK，ZDHHC19，CTPCT，APOD，Msp， CD71）；其次，根据筛选到的蛋白，构建了仔猪腹泻调控网络，并从中找到了MUC13基因参与的仔猪腹泻调控网络，该网络包括MUC13，MUC4，MUC1，MUC2，MUC3，TFF3，NOD1，TNFSF10，FGFR1，CDH2等10个基因；最后，采用DAVID数据库对图谱中的这10个基因进行基因功能注释分析，发现富集相关程度较高的一些基因聚类条目有上皮生长因子（ EGF）序列、SEA模块、跨膜结构域、胞质尾区等，因此推测MUC13基因结构的特异性决定了其在仔猪腹泻发展过程中具有特定的功能。文章建立的MUC13基因参与的仔猪腹泻调控网络具有一定的真实性，可以用来描述仔猪腹泻症发生发展过程中分子的相互作用。
위진일보천술자저복사발생적분자궤제，응용생물신식학방법구건료MUC13기인삼여적자저복사조공망락도보，병대기중적기인진행기인공능취류분석。수선，선택물충위저，통과STRING 수거고분석발현여MUC13상관련적유10충단백（MUC4，MUC20，LRCH3，FLJ33471，ACK，ZDHHC19，CTPCT，APOD，Msp， CD71）；기차，근거사선도적단백，구건료자저복사조공망락，병종중조도료MUC13기인삼여적자저복사조공망락，해망락포괄MUC13，MUC4，MUC1，MUC2，MUC3，TFF3，NOD1，TNFSF10，FGFR1，CDH2등10개기인；최후，채용DAVID수거고대도보중적저10개기인진행기인공능주석분석，발현부집상관정도교고적일사기인취류조목유상피생장인자（ EGF）서렬、SEA모괴、과막결구역、포질미구등，인차추측MUC13기인결구적특이성결정료기재자저복사발전과정중구유특정적공능。문장건립적MUC13기인삼여적자저복사조공망락구유일정적진실성，가이용래묘술자저복사증발생발전과정중분자적상호작용。
This study developed a regulation network of piglets diarrhea in which MUC13 gene participated and made an assay of gene functional annotation clustering .Firstly, 10 MUC13-associated proteins were generated through STRING 9.0 (MUC4, MUC20, LRCH3, FLJ33471, ACK, ZDHHC19, CTPCT, APOD, Msp, CD71).Secondly, using the 10 MUC13-associated proteins , we developed a regulation network of piglets diarrhea in which MUC13 gene participated, which included MUC13, MUC4, MUC1, MUC2, MUC3, TFF3, NOD1, TNFSF10, FGFR1, CDH2 genes.Finally, with DAVID bioinformatics resources , we made an assay of gene functional annotation clustering , and found specific functional annotations terms included domain:SEA, extracellular region , transmembrane protein , so we speculated that the specific structure of the MUC13 determined MUC13 had a specific function in the develop-ment process of piglet diarrhea .The network constructed in this study has certain authenticity and can be used for de-scribing molecular interactions in the occurrence and development of piglet diarrhea , which is important to elucidate the molecular mechanisms of diarrhea occurrence .