CAJ | 학술논문

利用TAS-ELISA方法对采自云南不同烟草种植区的710份花叶病样进行检测，其中546份样品与TMV抗体呈阳性反应。根据地理分布的差异，选择了38个代表性分离物进行TMV外壳蛋白基因的IC-RT-PCR扩增、克隆和测序。测序分析表明，38个TMV cp基因核苷酸序列相似性在95%~100%之间，其推导的氨基酸序列同源性为97.5%~100%。云南不同TMV分离物与TMV-U1、TMV-Fujian核苷酸相似性大于90%，其系统关系与TMV-U1和TMV-Fujian分离物聚为一簇，研究表明云南38个烟草分离物均属于TMV-U1株系。
이용TAS-ELISA방법대채자운남불동연초충식구적710빈화협병양진행검측，기중546빈양품여TMV항체정양성반응。근거지리분포적차이，선택료38개대표성분리물진행TMV외각단백기인적IC-RT-PCR확증、극륭화측서。측서분석표명，38개TMV cp기인핵감산서렬상사성재95%~100%지간，기추도적안기산서렬동원성위97.5%~100%。운남불동TMV분리물여TMV-U1、TMV-Fujian핵감산상사성대우90%，기계통관계여TMV-U1화TMV-Fujian분리물취위일족，연구표명운남38개연초분리물균속우TMV-U1주계。
710 samples of tobacco plants showing mosaic symptom were collected from different tobacco-growing areas in Yunnan province. 546 of the 710 samples were detected TMV antigen positive by TAS-ELISA. According to geographical distribution, 38 isolates were amplified, cloned and sequenced by IC-RT-PCR for TMV coat protein gene. Result showed that nucleotide sequences for coat protein of these 38 isolates were 95% to 100% similar while amino acid sequences were similar 97.5%~100%. The similarity among different TMV isolates in Yunnan and TMV-U1, TMV-Fujian were relatively high in nucleotide sequences of coat protein gene. The phylogenetic tree of TMV isolates showed TMV isolates in Yunnan share certain evolutionary relationship with TMV-U1 strain.