CAJ | 학술논문

以本实验室自行创制的云烟87八倍体（2n=8x=96）为母本，L-8四倍体为父本进行杂交，成功获得烟草六倍体（2n=6x=72）植株。经观察统计其花器官特征，花粉特征，并通过体外萌发及杂交试验对其育性进行初步研究。结果显示：烟草六倍体花蕾和花药明显大于云烟87四倍体×L-8四倍体杂交组合四倍体（简称四倍体杂种）后代的花蕾和花药，但花粉量较少；其花粉体外萌发率仅9.78%，明显低于四倍体杂种；六倍体花粉在六倍体及云烟87四倍体柱头上只极少量萌发并穿过花柱；以六倍体为父本，与云烟87四倍体烟草品种杂交以及六倍体自交，不能坐果；而以六倍体为母本、云烟87四倍体为父本可得到一定量种子，其单果种子数与杂种四倍体作母本、云烟87四倍体为父本时差异不大，但发芽率只有21.2%。这表明本研究中的六倍体植株作父本不育，而作母本具有一定的育性，后续研究利用中可将其用作母本。
이본실험실자행창제적운연87팔배체（2n=8x=96）위모본，L-8사배체위부본진행잡교，성공획득연초륙배체（2n=6x=72）식주。경관찰통계기화기관특정，화분특정，병통과체외맹발급잡교시험대기육성진행초보연구。결과현시：연초륙배체화뢰화화약명현대우운연87사배체×L-8사배체잡교조합사배체（간칭사배체잡충）후대적화뢰화화약，단화분량교소；기화분체외맹발솔부9.78%，명현저우사배체잡충；륙배체화분재륙배체급운연87사배체주두상지겁소량맹발병천과화주；이륙배체위부본，여운연87사배체연초품충잡교이급륙배체자교，불능좌과；이이륙배체위모본、운연87사배체위부본가득도일정량충자，기단과충자수여잡충사배체작모본、운연87사배체위부본시차이불대，단발아솔지유21.2%。저표명본연구중적륙배체식주작부본불육，이작모본구유일정적육성，후속연구이용중가장기용작모본。
Tobacco hexaploid plants were obtained successfully by crossingYunyan87 octoploids (2n=8x=96) with L-8 tetraploids, with octoploids as maternal parent. Fertility was studied with traits of flower and pollen, pollen germination in vitro and cross experiment. Results showed that:flower buds and anthers of hexaploid tobaccos were bigger than those of tetraploid tobaccos, with less amount of pollen. Under in vitro conditions, pollen germination rate of hexaploids was only 9.78%, significantly lower than that of tetraploid. Only a very small amount of hexaploid pollen could germinate on Yunyan87 stigma. There was no fruit when hexaploid as male parent while only some mature seeds were harvested when hexaploid as maternal parent. Germination rate of these seeds was only 21.2%. Our data suggested that hexaploid tobacco plants was male sterile and could be used as female parent in future breeding and genetic study.