CAJ | 학술논문

采用组织分离法和稀释平板法对西南桦猝倒病发生区健康苗木组织、根际土壤及咖啡壳堆肥化基质进行菌种分离和分子鉴定。将分离获得的17株菌株与西南桦苗木猝倒病病原菌尖孢镰刀菌和腐皮镰刀菌进行平板对峙实验，筛选病原拮抗菌。结果表明，筛选出的真菌5＃、7＃及细菌 BJ、 JC1对西南桦苗木猝倒病原菌尖孢镰刀菌和腐皮镰刀菌具有较强抑制效果。其中真菌5＃和7＃的抑菌率分别为91.30％和90.12％；细菌BJ和JC1抑菌带较宽，对峙4天分别达0.4 cm和0.5 cm，抑菌率分别为80.4％和78.6％，且BJ抑菌效果更好；真菌与细菌比较，拮抗真菌5＃和7＃比拮抗细菌BJ和JC1抑制热区西南桦苗木猝倒病菌效果更好。通过对峙实验筛选出的5＃、7＃、 BJ和JC1拮抗菌，对热区防治苗木猝倒病及生防制剂的开发应用具有参考价值。
채용조직분리법화희석평판법대서남화졸도병발생구건강묘목조직、근제토양급가배각퇴비화기질진행균충분리화분자감정。장분리획득적17주균주여서남화묘목졸도병병원균첨포렴도균화부피렴도균진행평판대치실험，사선병원길항균。결과표명，사선출적진균5＃、7＃급세균 BJ、 JC1대서남화묘목졸도병원균첨포렴도균화부피렴도균구유교강억제효과。기중진균5＃화7＃적억균솔분별위91.30％화90.12％；세균BJ화JC1억균대교관，대치4천분별체0.4 cm화0.5 cm，억균솔분별위80.4％화78.6％，차BJ억균효과경호；진균여세균비교，길항진균5＃화7＃비길항세균BJ화JC1억제열구서남화묘목졸도병균효과경호。통과대치실험사선출적5＃、7＃、 BJ화JC1길항균，대열구방치묘목졸도병급생방제제적개발응용구유삼고개치。
The tissue isolation and dilution-plate were used on healthy seedlings tissue , rhizosphere soil and coffee shell composting matrix for isolating antagonists of damping-off disease of Betula alnoides seedlings;and the isolated strains were identified through molecular method .The confront culture was conducted between 17 isolated strains and the pathogens Fusarium oxysporum and Fusarium solani to screen for antagonists .The results indicated that , the fungus strains 5#, 7#and bacteria BJ and JC1showed promising inhibition effects on two pathogens .The inhibi-tion rates of fungus strains 5# and 7# to the pathogens were 91.30 % and 90.12 % respectively , two bacteria strains had the inhibition belts of 0.4 cm and 0.5 cm, with the inhibition rates of 80.40%and 78.60% respec-tively , and BJ showed the better inhibition effect .The inhibition effects of two isolated fungal strains were better than bacterial strains .