CAJ | 학술논문

从EMBL数据库下载珍珠鸟（ Taeniopygia guttata） Z染色体全部基因的cDNA序列，共有610个基因的CDS序列纳入统计分析。使用CodonW （1．4．2）对CDS序列进行密码子偏性研究，确定了UUC、CUC、CUG等28个密码子为珍珠鸟Z染色体基因表达的“最优”密码子。对应分析表明，影响珍珠鸟Z染色体基因表达的密码子用法的主要因素分别为GC3s（同义密码子第3位的G C频率）、CDS的GC含量以及基因的表达水平。据此推论，珍珠鸟Z染色体基因表达的密码子偏性是核苷酸组成和选择等因素综合作用的结果。
종EMBL수거고하재진주조（ Taeniopygia guttata） Z염색체전부기인적cDNA서렬，공유610개기인적CDS서렬납입통계분석。사용CodonW （1．4．2）대CDS서렬진행밀마자편성연구，학정료UUC、CUC、CUG등28개밀마자위진주조Z염색체기인표체적“최우”밀마자。대응분석표명，영향진주조Z염색체기인표체적밀마자용법적주요인소분별위GC3s（동의밀마자제3위적G C빈솔）、CDS적GC함량이급기인적표체수평。거차추론，진주조Z염색체기인표체적밀마자편성시핵감산조성화선택등인소종합작용적결과。
The gene cDNA sequences of Z chromosome in zebra finch ( Taeniopygia guttata) were down-loaded from EMBL database .Total 610 CDS sequences were finally included in analyses .By the use of CodonW (1.4.2),28 codons (eg.UUC,CUC,CUG et al.) were identified as the optimal codons .The corresponding ana-lyses indicted that the main factors influcing codon usage of genes of Z chromosome in zebra finch were GC 3s, GC content of CDS ,and gene expression level .It can be deduced that the codon usage of Z chromosome in ze-bra finch is most likely affected by the combination of nucleotide composition and selection and so on .