CAJ | 학술논문

为研究临床上仔猪黄痢大肠杆菌的耐药性与整合子之间是否存在一定的相关性，从仔猪黄痢临床病例中分离鉴定了两株大肠杆菌，采用K-B法检测这两株菌对21种抗菌药物的敏感性，并对I类整合酶和qacE△1-sull基因进行PCR检测。结果表明：这两株大肠杆菌只对氯霉素敏感或中介，对其他20种药物耐药。 PCR检测结果表明这两株菌均能扩增出I类整合酶基因和qacE△1-sull基因，且这两株菌均携带了几个大小不同的基因盒，为从基因水平上了解大肠杆菌的耐药性提供了一定的参考。
위연구림상상자저황리대장간균적내약성여정합자지간시부존재일정적상관성，종자저황리림상병례중분리감정료량주대장간균，채용K-B법검측저량주균대21충항균약물적민감성，병대I류정합매화qacE△1-sull기인진행PCR검측。결과표명：저량주대장간균지대록매소민감혹중개，대기타20충약물내약。 PCR검측결과표명저량주균균능확증출I류정합매기인화qacE△1-sull기인，차저량주균균휴대료궤개대소불동적기인합，위종기인수평상료해대장간균적내약성제공료일정적삼고。
Class1 integron is strongly associated with multidrug resistance in bacteria .In this study , the drug sensitivity of the pathogenic E.coli strains isolated from piglets to 21 antimicrobial agents was tested by the Kirby-Bauer method,and the genes of Class 1 Integron and qacE△1-sul1 were detected by PCR.The results showed that the isolates were only sensitive to chloramphenicol ,and resistant to the other 20 drugs.The isolates were positive for intI1 and qacE△1-sul1 gene,and the detect to intI1 gene cassette showed that the isolates harbored some kinds of gene sequens .The result suggests that it is critical to monitor drug resistant bacteria at the gene level .